Clarification of mechanism of division, proliferation, differentiation, and apoptosis of supporting cells in regeneration of cochlear hair cells

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In developing rats, double-staining with antibodies to Caspase 3 and p27 have shown that large numbers of positive staining cells exist in the organ of Corti on 7 days after birth. These findings suggest that apoptosis is promoted and that proliferation was stopped 7 days after birth. The organ of Corti extirpated on 7th day after birth was cultured with 1mM neomycin solution or acidic acid for 5 days. Then, bromodeoxyuridine (BrdU) as a proliferation marker was administered from 3rd day after starting the culture. No BrdU positive staining cell was observed in the supporting cell, suggesting that supporting cells did not proliferate up to 3^<rd> day after missing hair cell.