Clarification of mechanism of division, proliferation, differentiation, and apoptosis of supporting cells in regeneration of cochlear hair cells

阐明耳蜗毛细胞再生中支持细胞的分裂、增殖、分化和凋亡机制

• 批准号: 20591973
• 负责人: SUZUKI Mitsuya
• 金额: $ 3.08万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20591973/
• 关键词: 未成熟ラット; 有毛細胞; 支持細胞; 増殖; アポトーシス; BrdU; 有毛細胞消失; 蝸牛; ネオマイシン; 分裂; 未成熟ラツト; Caspase3; 27; 半規管膨大部

In developing rats, double-staining with antibodies to Caspase 3 and p27 have shown that large numbers of positive staining cells exist in the organ of Corti on 7 days after birth. These findings suggest that apoptosis is promoted and that proliferation was stopped 7 days after birth. The organ of Corti extirpated on 7th day after birth was cultured with 1mM neomycin solution or acidic acid for 5 days. Then, bromodeoxyuridine (BrdU) as a proliferation marker was administered from 3rd day after starting the culture. No BrdU positive staining cell was observed in the supporting cell, suggesting that supporting cells did not proliferate up to 3^<rd> day after missing hair cell.

在发育中的大鼠中，Caspase 3 和 p27 抗体双重染色显示出生后 7 天的 Corti 器官中存在大量阳性染色细胞。这些发现表明，出生后 7 天细胞凋亡得到促进，增殖停止。出生后第7天摘除的Corti器官用1mM新霉素溶液或酸性酸培养5天。然后，从开始培养后第3天开始施用作为增殖标记物的溴脱氧尿苷(BrdU)。在支持细胞中没有观察到BrdU阳性染色细胞，表明支持细胞在缺失毛细胞后直至第3天都没有增殖。

项目成果

Induction of hair cell regeneration via the proliferation of supporting cell.

通过支持细胞的增殖诱导毛细胞再生。

• 发表时间: 2008
• 作者: Takashi Matsumoto;et al.;Mitsuya Suzuki
• 通讯作者: Mitsuya Suzuki

Effect of Burow's solution on the basement membrane anionic sites in the stria Vascularis.

Burow 溶液对血管纹基底膜阴离子位点的影响。

• 发表时间: 2010
• 作者: Mitsuya Suzuki;Hitoshi Iwamura;Takashi Sakamoto;Akinori Kashio
• 通讯作者: Akinori Kashio

Effect of Burow's solution in the guinea pig inner ear.

Burow 溶液对豚鼠内耳的影响。

• 发表时间: 2010
• 期刊: Ann Otol.Rhinol.Laryngol. 119
• 作者: Suzuki M;Kashio A;Sakamoto T;Yamasoba T
• 通讯作者: Yamasoba T

Changes in the structure and function of the inner ear caused by Burow's solution

Burow溶液引起内耳结构和功能的变化

• 发表时间: 2009
• 作者: Mitsuya Suzuki;Akinori Kashio;Takashi Sakamoto;Tatsuya Yamasoba
• 通讯作者: Tatsuya Yamasoba