Roles of a cell membrane-nuclear shuttle molecule Hic-5 in skin wound healing

细胞膜-核穿梭分子 Hic-5 在皮肤伤口愈合中的作用

• 批准号: 23592647
• 负责人: INUI Shigeki
• 金额: $ 3.24万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23592647/
• 关键词: 創傷治癒学; メラノサイト; Hic-5; 核内因子; 増殖; 運動; 浸潤; 細胞増殖因子; 線維芽細胞; ケラチノサイト; 創傷治癒; 表皮; 皮膚再生; 分化

The immunocytochemistry revealed that Hic-5 shifts from peri-nuclei to cell membrane during keratinocyte differentiation. Using a stable knockdown HaCat keratinocytes (Hic-5KD-HaCat) and control HaCat (cHaCat), in vitro assays revealed that Hic-5 knockdown suppresses DNA synthesis and accelerates migration more than cHaCat. During HaCat keratinocyte differentiation, keratin 1 expression is suppressed in Hic-5KD-HaCat. Regarding adhesion ability to type IV collagen-coated plates, it was suppressed by Hic-5 knockdown. From these results, Hic-5 plays multipotential functions in growth, differentiation, migration and adhesion of human keratinocytes in wound healing. The 16-F1 murine melanoma cells as a model cell line for normal melanocytes were studied likewise, reveling the potential roles of Hic-5 in the proliferation and motility, suggesting its roles in re-pigmentation during wound healing.

免疫细胞化学显示，在角质形成细胞分化过程中，Hic-5从核周向细胞膜转移。使用稳定的敲除HaCat角质形成细胞(Hic-5KD-HaCat)和对照HaCat(CHaCat)的体外实验表明，Hic-5基因敲除比cHaCat更能抑制DNA合成和促进迁移。在HaCat角质形成细胞分化过程中，角蛋白1在Hic-5KD-HaCat中的表达受到抑制。对于IV型胶原涂层钢板的黏附能力，Hic-5基因敲除后被抑制。这些结果表明，Hic-5在创伤愈合过程中对人角质形成细胞的生长、分化、迁移和黏附具有多方面的潜在作用。16-F1小鼠黑色素瘤细胞作为正常黑素细胞的模型细胞，揭示了Hic-5在黑素细胞增殖和运动中的潜在作用，提示其在伤口愈合过程中重新着色的作用。

项目成果

Dermoscopic evaluation of erythema associated with pressure ulcers

• DOI: 10.1111/j.1365-4632.2010.04784.x
• 发表时间: 2011-08-01
• 期刊: INTERNATIONAL JOURNAL OF DERMATOLOGY
• 影响因子: 3.6
• 作者: Inui, Shigeki;Ikegawa, Hitoshi;Itami, Satoshi
• 通讯作者: Itami, Satoshi

Trichoscopy (Scalp dermoscopy) as a new methodology for diagnosing and observing hair loss diseases

毛发镜检查（头皮皮肤镜检查）作为诊断和观察脱发疾病的新方法

• 发表时间: 2012
• 作者: Sato T;Ichioka S;加王 文祥;Inui S
• 通讯作者: Inui S

円形脱毛症における睡眠障害の調査

斑秃睡眠障碍的调查

• 发表时间: 2011
• 作者: 乾重樹;戸田直歩;板見智
• 通讯作者: 板見智

Cartilage oligomeric matrix protein as matrix deposition enhancer in keloid : Microarray approach and in vivo and in vitro evidence

软骨寡聚基质蛋白作为瘢痕疙瘩基质沉积增强剂：微阵列方法和体内外证据

• 发表时间: 2011
• 作者: Inui S;Shono F;Nakajima T;Hosokawa K;Itami S
• 通讯作者: Itami S

I .腋臭症・多汗症のメカニズム, 2.腋臭(わきが臭)とはどういうものか?臭いの生じる原因.「腋臭症・多汗症治療実践マニュアル」(細川亙、坂井靖夫編)

一、腋臭和多汗症的机理、二、什么是腋臭（腋臭）？产生臭味的原因《腋臭和多汗症治疗实用手册》（细川涉、酒井康夫主编）

• 发表时间: 2012
• 作者: 乾重樹;板見智:
• 通讯作者: 板見智: