Roles of a cell membrane-nuclear shuttle molecule Hic-5 in skin wound healing

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The immunocytochemistry revealed that Hic-5 shifts from peri-nuclei to cell membrane during keratinocyte differentiation. Using a stable knockdown HaCat keratinocytes (Hic-5KD-HaCat) and control HaCat (cHaCat), in vitro assays revealed that Hic-5 knockdown suppresses DNA synthesis and accelerates migration more than cHaCat. During HaCat keratinocyte differentiation, keratin 1 expression is suppressed in Hic-5KD-HaCat. Regarding adhesion ability to type IV collagen-coated plates, it was suppressed by Hic-5 knockdown. From these results, Hic-5 plays multipotential functions in growth, differentiation, migration and adhesion of human keratinocytes in wound healing. The 16-F1 murine melanoma cells as a model cell line for normal melanocytes were studied likewise, reveling the potential roles of Hic-5 in the proliferation and motility, suggesting its roles in re-pigmentation during wound healing.