Usefulness on detection of intracanal microbe with photodynamic agent and laser

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Methylene blue as a photosensitizer, and poly (lactic-co-glycolic acid) (PLGA) spherical nanoparticles loaded with chitosan (particle size 250 nm) were used to combine microbe in this study. DIAGNOdent laser device (wavelength 655 nm) was used to detect photosensitizer with nanoparticles combined to microbe, and this study was performed within the nanoparticles concentration range of 0.01, 0.1, 1, 10 mg/ml. Detection sensitivity was slight increased using methylene blue-coated PLGA nanoparticles, but some problems still remain to complete diagnosis.