Molecular histological analysis on calcification of alveolar bone during healing process of chronic periapical lesion.

对他的影响：YAG激光辐射和根运河治疗在使用Wistar大鼠（8周老，重量200- 300克）的周期性课程中进行了根运河治疗评估。在根运河离开1个月后，根运河在30秒内受到辐射：YAG激光器在2赫兹和34、68和102毫焦/脉冲下。非辐射运河由格罗斯曼提供控制的传统方法准备。在氢钙被放入根运河作为医学和根运河被密封在复合树脂中，激光辐射对医学区域的周期性薄膜辐射的影响在0、2天、1、2和4周后在光照显微镜后H·E、TRAP和ALP冻结下进行了评估。治疗后4周，无炎症或复位可在控制组或34毫焦/脉冲辐射组中观察到任何病例。在两个小组之间，治疗过程没有明显的差异。由于根表面的分解，在102毫焦/脉冲辐射组中观察到4个病例（80%）的4个病例（80%），在控制方面明显不同（P<0.01）。在另一方面，在某些内部医学中，治疗过程中没有明显的区别。这些结果建议根据Er：受感染的根运河上的YAG激光辐射是最小的，如果合适的参数是选定的，则根据Er：根运河上的周期性问题的热效应。在进一步的研究中，将需要超过4周的观察期。骨胶原蛋白的作用是计算过程中的标记，任何用于加速计算的药物都将被调查。

The effects of Er : YAG laser irradiation and root canal medication on the periapical lesion during root canal treatment were evaluated using Wistar rats (8 weeks old and weighing 200-300g) histopathologically. After root canals were left unsealed for 1 month, root canals were irradiated using an Er:YAG laser at 2 Hz and 34, 68, and 102 mJ/pulse for 30 seconds. Non-irradiated canals prepared by a conventional method of Grossman served as controls. After calcium hydroxide was put into root canals as medication and root canals were sealed with composite resin, the effects of laser irradiation on the periodontal tissues at apical area were evaluated at 0, 2 days, 1, 2, and 4 weeks after irradiation under light microscopy after H・E, TRAP, and ALP staining. At 4 weeks after treatment, no inflammation or resorption was observed in any cases in the control or 34 mJ/pulse-irradiated groups. There was no significant difference of healing process between 2 groups. However, moderate to severe inflammation with resorption of root surface was observed in 4 of 5 cases (80%) in the 102 mJ/pulse-irradiated group, which was significantly different from the control (P<0.01). On the other hand, there was no significant difference of healing process among some intracanal medicaments. These results suggest that thermal effects on periapical tissues during root canal preparation by Er:YAG laser irradiation at infected root canals are minimal, if appropriate parameters are selected. Observation period of longer than 4 weeks will be needed in further research. A role of osteopontin as a marker on calcification process and any medicaments to accelerate calcification will be investigated.