Optogenetic approach of gene regulation

点击翻译按钮获取中文摘要

In order to generate photoactivatable Cre, we made a series of fusion proteins between LOV domain and Cre and introduce into HEK cells along with lox-stop-lox-GFP, which allows detection of Cre activity by GFP. However, irrespective illumination, we saw GFP signal, indicating that LOV domain did not effectively suppress the activity of Cre. However, in some of constructs, GFP was dimmer than others. We will further pursue these constructs to see if we can improve them by adding additional mutations.