Analysis of genome inactivation mechanism using chromosomal site-specific gene integration

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In this study, we first devised a site-specific gene integration method, and established two female mouse lines, whose two X chromosomes carry different fluorescent protein genes. X chromosome inactivation phenomenon can be visualized using these mouse lines. Processes of X inactivation and reactivation can be analyzed in living cells of these mouse lines for the first time.Using female somatic cells isolated from hybrid individuals between Xist knockout mice and asian mouse subspecies, ChIP-Seq analysis of histone modifications has been conducted. Differences in epigenetic states between inactive X and active X has been revealed.