Analysis of archaeal viruses infecting hyperthermophilic archaea

感染超嗜热古菌的古菌病毒分析

• 批准号: 23651208
• 负责人: YOSHIMUNE Kazuaki
• 金额: $ 1万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651208/
• 关键词: 古細菌; 超好熱古細菌; 高温温泉; アーキウィルス; ウィルス DNA; クローン ライブラリ; DNAシーケンス解析

Archaea belongs to a major domain of life which is distinct from Eukaryota and Eubacteria. Some unique enzymes from Archaea are applied for biotechnology. Since useful enzymes for gene manipulation often derived from viruses, archaeal viruses may have these useful enzymes. However, only a few viruses infecting archaea are reported, especially those infecting hyperthermophilic archaea are scarcely reported. Here, viruses infecting hyperthermophilic archaea are collected from hot springs having temperatures of more than 80?C where hyperthermophilic archaea are living. Hot spring water having temperatures of near 100 ?C in Beppu, Atagawa and Toyako was collected. The obtained water was filtered with 0.22 μm filters and impurities were removed. Viruses in the water were concentrated with 50kDa-filters. DNA was extracted and purified with protease treatment and phenol/chloroform extraction. The purified DNA was subcloned and the DNA sequences were determined to analyze their function.

古生菌属于与真核生物和真细菌不同的一个主要生命领域。古细菌中一些独特的酶被应用于生物技术。由于基因操作的有用酶通常来源于病毒，古细菌病毒可能具有这些有用的酶。然而，感染古细菌的病毒报道很少，特别是感染超嗜热古细菌的病毒报道很少。在这里，感染超嗜热古细菌的病毒是从温度超过80度的温泉中收集的。C是超嗜热古细菌生存的地方。温泉水的温度接近100度？C在别府、Atagawa和Toyako被收集。得到的水用0.22 μm过滤器过滤，去除杂质。水中的病毒用50kda过滤器浓缩。采用蛋白酶处理和苯酚/氯仿萃取法提取纯化DNA。对纯化的DNA进行亚克隆，并测定DNA序列，分析其功能。

项目成果

Thermophilic Microbes in Environmental and Industrial Biotechnology

环境和工业生物技术中的嗜热微生物

• 发表时间: 2013
• 作者: Jiro Nakayama;Jiahui Jiang;Koichi Watanabe;Kangting Chen;Huang Ninxin;Kazunori Matsuda;Takashi Kurakawa;Hirokazu Tsuji;Kenji Sonomoto;Yuan-Kun Lee;K. Yoshimune and Y. Kawarabayasi
• 通讯作者: K. Yoshimune and Y. Kawarabayasi

Thermophilic Microbes in Environmental and Industrial Biotechnology.

环境和工业生物技术中的嗜热微生物。

• 发表时间: 2013
• 作者: 吉 宗 一 晃;他
• 通讯作者: 他