Dissociated neuronal culture of rats expressing insect odorant receptors as an odorant sensor

表达昆虫气味受体作为气味传感器的大鼠的分离神经元培养物

基本信息

批准号：
23656174
负责人：
TAKAHASHI Hirokazu
金额：
$ 2.66万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2011
资助国家：
日本
起止时间：
2011 至无数据
项目状态：
已结题

项目摘要

Artificial odorant sensors generally perform poorer than olfactory systems in living organisms. The excellent performances of living odorant systems are achieved by the molecular recognition abilities of odorant receptors and the neuronal information processing that follows. To take advantages of this, here we propose a novel hybrid odorant biosensor by means of expressing ionotropic odorant receptors of insects into dissociated neuronal cultures of rodents. This combination of materials brings significant advantages such as easy functional expression, prolonged lifetime, and an ability to amplify the weak ionic currents of odorant receptors. In the present work, pheromone receptors and co-receptors of silkmoth, i. e., BmOR1 and BmorOrco, were expressed in neuronal cultures via liposome transfection. Consequently, BmOR1 and BmorOrco were co-expressed in 8% of neuronal cells, and both receptors were co-localized on a cell membrane. In Ca++imaging experiments, synchronous increase of calcium signals at the presentation of BOL was found in both transfected cells and non-transfected cells in a dose-dependent manner. These results provide the proof-of-concept of the proposed hybrid odorant biosensor.

人造气味传感器通常比活生物体中的嗅觉系统表现较差。生物气味系统的出色性能是通过气味受体的分子识别能力以及随后的神经元信息处理来实现的。为了利用这一点，我们在这里提出了一种新型的混合气味生物传感器，通过将昆虫的离子性气味受体表达为啮齿动物的解离神经元培养物。这种材料的组合带来了显着的优势，例如易于功能表达，寿命延长以及放大气味受体弱离子电流的能力。在目前的工作中，丝绸的信息素受体和共受体，i。例如，Bmor1和Bmororco通过脂质体转染在神经元培养物中表达。因此，在8％的神经元细胞中共表达BMOR1和BMORORCO，并且两个受体在细胞膜上共定位。在CA ++成像实验中，在转染的细胞和未转染的细胞中以剂量依赖性方式发现了钙信号的同步增加。这些结果提供了拟议的混合气味生物传感器的概念概念。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The feasibility study of novel odor biosensor using dissociate neuronal culture expressing ion channel built-in odorreceptors

使用表达离子通道内置气味感受器的分离神经元培养物新型气味生物传感器的可行性研究

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Norio Tanada;Takeshi Sakurai;Hidefumi Mitsuno;Douglous Bakkum;Ryohei Kanzaki;Hirokazu Takahashi
通讯作者：
Hirokazu Takahashi

Feasibility study of odor biosensor using dissociate neuronal culture with gene expression of ionotropic odorant receptors

使用离解神经元培养与离子型气味受体基因表达的气味生物传感器的可行性研究

DOI：
发表时间：
2011
期刊：
影响因子：
0
作者：
Norio Tanada;Takeshi Sakurai;Hidefumi Mitsuno;Douglas Bakkum;Ryohei Kanzaki;Hirokazu Takahshi
通讯作者：
Hirokazu Takahshi

Light-addressed single-neuron stimulation in dissociated neuronal cultures with sparse expression of ChR2

DOI：
10.1016/j.biosystems.2011.10.002
发表时间：
2012-02
期刊：
Bio Systems
影响因子：
0
作者：
Hirokazu Takahashi;Takeshi Sakurai;Hideo Sakai;D. Bakkum;J. Suzurikawa;R. Kanzaki
通讯作者：
Hirokazu Takahashi;Takeshi Sakurai;Hideo Sakai;D. Bakkum;J. Suzurikawa;R. Kanzaki

The feasibility study of novel odor biosensor using dissociate neuronal culture expressing ion channel built-in odor receptors

使用表达离子通道内置气味受体的分离神经元培养物新型气味生物传感器的可行性研究