The identification of molecular targets for glioblastoma using mouse model transplanted transgenic neural stem cell

利用小鼠模型移植转基因神经干细胞鉴定胶质母细胞瘤的分子靶点

• 批准号: 23791588
• 负责人: TAMASE Akira
• 金额: $ 2.66万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791588/
• 关键词: グリオーマ; 幹細胞; マウスモデル; 標的分子; マイクロアレイ; 分子標的

We explored the genes which overexpressed in glioma stem-like cells using the mouse glioma model transplanted transgenic neural stem cells. Glioma stem-like cells were labeled by Green Fluorescent Protein (GFP). The gene expression profiles of which cells are GFP positive or are GFP negative havebeen obtained by microarray data, respectively. Fifty candidate genes were picked up. Among them, two genes have been identified as promising genes.

我们利用移植转基因神经干细胞的小鼠脑胶质瘤模型，探索了在胶质瘤干细胞中高表达的基因。用绿色荧光蛋白(GFP)标记胶质瘤干细胞。通过微阵列数据分别获得了GFP阳性和GFP阴性细胞的基因表达谱。挑选了50个候选基因。其中，有两个基因被确定为有希望的基因。