The elucidation of new mechanosensor in oral mucosal epithelium

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I hypothesized that TRPV4 (Transient receptor potential cation channel subfamily V member 4) channels in oral mucosal epithelium have sensitivity of hypoosmolarity from saliva, and support the homeostasis of oral epithelial structure. I investigated the sensitivity of hypoosmolarity and it’s function using rats or TRPV4 knockout mice. I cultured primary buccal epithelial cells and confirmed TRPV4 expression in mRNA level and protein level. I took advantage of electrophysiological and Ca2+ imaging methods, and found TRPV4 induced currents and Ca2+ mobility using TRPV4 activators and hypotonic stimulation. With the same stimuli, there was a rise of ATP released from oral epithelial cells, which suggested ATP to be a mediator to peripheral nerves. I used the method of immunocytochemistry and found hypoosmolarity induced reorganization of actin, a kind of cytoskeleton. This suggested that TRPV4 supports intercellular contact