The construction of multi-chromatin inducible human artificial chromosome (HAC)

多染色质诱导型人类人工染色体（HAC）的构建

• 批准号: 23770011
• 负责人: NAKANO Megumi
• 金额: $ 2.91万
• 依托单位: Kazusa DNA Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770011/
• 关键词: セントロメア; 人工染色体; クロマチン; ヒト人工染色体; 染色体; HAC

At the human centromere, various chromatins (e.g. centromere chromatin, heterochromatin) assemble on the highly repetitive DNA (alphoid DNA). Is has been suggested that each chromatin is important for centromere functions, however, the accumulation mechanism of different chromatins on the same DNA and the mechanism of maintenance and regulation of centromere activity are still unknown. From the analyses of a human artificial chromosome (HAC) containing tetracycline operator (tetO), it has been suggested that the balance between various chromatins on centromere directly affected the centromere activity. To analyze the roles of chromatins in detail, we constructed another synthetic alphoid DNA containing lactose operator (lacO). On the tandem array of tetO / lacO centromere DNA, various chromatin modifications could be induced individually by the tethering of tetR- or lacI- fusion proteins.

在人的着丝粒上，各种染色质（如着丝粒染色质、异染色质）聚集在高度重复的DNA（α DNA）上。研究表明，每个染色质对着丝粒的功能都很重要，但不同染色质在同一DNA上的聚集机制以及着丝粒活性的维持和调节机制仍不清楚。对一条含有四环素操纵基因tetO的人工染色体的分析表明，着丝粒上各种染色质之间的平衡直接影响着着丝粒的活性。为了详细分析染色质的作用，我们构建了另一个人工合成的含乳糖操纵基因的α DNA（lacO）。在tetO / lacO着丝粒DNA的串联阵列上，可以通过tetR-或lacI-融合蛋白的拴系单独诱导各种染色质修饰。

项目成果

The analyses of regulation and maintenance mechanisms of centromere functional structure with chromatin-alterable human artificial chromosome system

染色质可变人人工染色体系统对着丝粒功能结构的调控和维持机制分析

• 发表时间: 2012
• 作者: Megumi Nakano;Jun-Ihirou Ohzeki;Koichiro Otake;Nobuaki Shono;Vladimir Larionov;William Earnshaw;and Hiroshi Masumoto
• 通讯作者: and Hiroshi Masumoto

Organization of Synthetic Alphoid DNA Array in Human Artificial Chromosome (HAC) with a Conditional Centromere

• DOI: 10.1021/sb3000436
• 发表时间: 2012-12-01
• 期刊: ACS SYNTHETIC BIOLOGY
• 影响因子: 4.7
• 作者: Kouprina, Natalay;Samoshkin, Alexander;Larionov, Vladimir
• 通讯作者: Larionov, Vladimir