Analysis of the diversity of fungal endophytes with metagenomics

利用Roche 454焦磷酸测序技术，对亚洲和澳大利亚两个低地热带森林和一个亚热带森林的6种树木叶片内生真菌的α多样性和分类组成进行了研究。将总共95，278个真菌读段以97%相似性阈值聚类成224个OTU。在鉴定的178个OTU中，分别有148个和30个OTU属于子囊菌门和担子菌门。主要OTU是常见的叶片内生菌和病原菌。同一树种叶片内生真菌群落的相似性较低，表明其β多样性较高。

The alpha diversity and taxonomic composition of endophytic fungi associated with leaves of six tree species were examined in two lowland tropical forests and a subtropical forest in Asia and Australia, using ITS metabarcoding with Roche 454 pyrosequencing. A total of 95,278 fungal reads were clustered into 224 OTUs at 97% similarity threshold. Of the 178 OTUs identified to the phylum, 148 and 30 OTUs belonged to Ascomycota and Basidiomycota, respectively. Major OTUs were common foliar endophytes and pathogens. The low similarities of endophytic fungal assemblages between leaf samples of a single tree species suggested high beta diversity.