课题基金 / 基金详情

Structural analysis of myosin II subfragment 1 decorated actin filament by CryoEM

Structural analysis of myosin II subfragment 1 decorated actin filament by CryoEM
通过 CryoEM 对肌球蛋白 II 亚片段 1 修饰的肌动蛋白丝进行结构分析
批准号：
23770179
负责人：
FUJII Takashi
金额：
$2.91万
依托单位：
The Institute of Physical and Chemical Research (2012)Osaka University (2011)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2011
资助国家：
日本
项目状态：
已结题
起止时间：
2011 至 2012
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23770179/
关键词：
低温電子顕微鏡法アクチンミオシン筋肉

项目摘要

项目成果

FUJII Takashi的其他基金

相似基金

相关文献

中文摘要
翻译
点击翻译按钮获取中文摘要
英文摘要
To understand the mechanism of muscle contraction,it is necessary to reveal how myosin binds to actin. Despite tons of biochemical information, the detail structure of actin-myosin complex remains unknown. We have obtained 7Å resolution density map of actin-myosin II S1 rigor complex by CryoEM. The density map clearly resolves all the secondary structures, such as α-helices, β-structures and loops, having made unambiguous modeling and refinement possible. The structure suggests that myosin undergoes a drastic conformational change upon strongly binding to the actin filament and that a myosin binds to two actin monomers. The high resolution structure gave us a relative three dimensional arrangement between actin and myosin, suggesting that the mutations caused myopathic illness is highly related to the residues forming the actin-myosin interface.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
High resolution structural analysis of the actin – myosin rigor complex by CryoEM.
通过 CryoEM 对肌动蛋白 - 肌球蛋白严格复合物进行高分辨率结构分析。
DOI： --
发表时间： 2012
期刊：
影响因子： --
作者： [Fujii, T., et al]
通讯作者： et al
Structure of a type III secretion needle at 7-Å resolution provides insights into its assembly and signaling mechanisms
DOI： 10.1073/pnas.1116126109
发表时间： 2012-03-20
期刊： PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子： 11.1
作者： [Fujii, Takashi, Cheung, Martin, Namba, Keiichi]
通讯作者： Namba, Keiichi
A bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation.
DOI： 10.1126/science.1229091
发表时间： 2012-12-07
期刊： Science (New York, N.Y.)
影响因子： --
作者： [Gayathri P, Fujii T, Møller-Jensen J, van den Ent F, Namba K, Löwe J]
通讯作者： Löwe J
低温電子顕微鏡によるらせん複合体の高分解能構造解析
使用冷冻电子显微镜对螺旋复合物进行高分辨率结构分析
DOI： --
发表时间： 2012
期刊：
影响因子： --
作者： [Fujii, T., et a1, 渕上壮太郎, 粕谷有造, 藤井高志]
通讯作者： 藤井高志
Structural analysis of the complex of F-actin and DNGR-1 by CryoEM.
Singing death: Death, emotion and emotion strategy described in Greek verse funerary inscriptions from western Asia Minor
  • 批准号：
    25884038
  • 项目类别：
    Grant-in-Aid for Research Activity Start-up
  • 资助金额：
    $1.91万
  • 财政年份：
    2013
  • 负责人：
    FUJII Takashi
  • 依托单位：
    Kyoto University
Rapid test of drying shrinkage strain of concrete
  • 批准号：
    24760348
  • 项目类别：
    Grant-in-Aid for Young Scientists (B)
  • 资助金额：
    $3.0万
  • 财政年份：
    2012
  • 负责人：
    FUJII Takashi
  • 依托单位：
    Okayama University
The ABC transporter family in psychiatric diseases
海外基金