Development of real-time secretion analysis of immune single cells
Development of real-time secretion analysis of immune single cells
批准号:
23770189
负责人:
SHIRASAKI Yoshitaka
金额:
$3.0万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
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英文摘要
We developed a platform for real-time secretion analysis at the single cell resolution. The platform enabled to monitor secretion dynamics of thousand of single cells with 30-min time resolution for over 8 hours. We also demonstrated multi-parametric measurement of secretion dynamics and cellular states with 1-min time resolution. We applied our platform to inflammatory responses of immune cells and observed intercellular and intracellular heterogeneity of cytokine secretion.
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単一細胞イムノアッセイによるNLRP3 体細胞モザイクの機能的解析の試み
尝试通过单细胞免疫分析对 NLRP3 体细胞嵌合体进行功能分析
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[樋口芳樹(発表者), 茶竹俊行, 横山武司, 山田太郎, 白崎 善隆]
通讯作者:
白崎 善隆
DOI:
10.1016/j.snb.2011.06.043
发表时间:
2011-11-28
期刊:
SENSORS AND ACTUATORS B-CHEMICAL
影响因子:
8.4
作者:
[Haneoka, Mai, Shirasaki, Yoshitaka, Funatsu, Takashi]
通讯作者:
Funatsu, Takashi
Fabrication of high-aspect-ratio amorphous perfluorinated polymer structure for total in ternal reflection fluorescence microscopy
用于全内反射荧光显微镜的高纵横比非晶全氟聚合物结构的制备
DOI:
10.1016/j.mee.2011.02.117
发表时间:
2011
期刊:
Microelectronic Engineering
影响因子:
2.3
作者:
[A.Nakahara, Y.Shirasaki, K.Kawai, O.Ohara, J.Mizuno]
通讯作者:
J.Mizuno
微細凹版法を用いたタンパク質高分泌株の高効率単一細胞スクリーニング
微凹版法高效单细胞筛选高蛋白分泌菌株
DOI:
--
发表时间:
2011
期刊:
ファルマシア
影响因子:
--
作者:
[茶竹俊行, 森本幸生, 白崎 善隆]
通讯作者:
白崎 善隆
Toward an understanding of immune cell sociology: real-time monitoring of cytokine secretion at the single-cell level
了解免疫细胞社会学:在单细胞水平实时监测细胞因子分泌
DOI:
10.1002/iub.1175
发表时间:
2012
期刊:
IUBMB Life
影响因子:
4.6
作者:
[Shirasaki Y, Yamagishi M, Shimura N, Hijikata A,Ohara O.]
通讯作者:
Hijikata A,Ohara O.
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Development of a micro cell sorter with thermal gelation polymer solution on a 3D focusing PDMS chip which fabricated using one step 3D casting technique
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批准号:20710103
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.83万
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财政年份:2008
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负责人:SHIRASAKI Yoshitaka
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依托单位:
海外基金