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Search for novel membrane trafficking pathway involved in autophagy

Search for novel membrane trafficking pathway involved in autophagy
寻找参与自噬的新型膜运输途径
批准号:
23770216
负责人:
ITOH Takashi
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012

项目摘要

项目成果

ITOH Takashi的其他基金

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相关文献

中文摘要
翻译
巨自噬(Macroautpohagy,以下简称自噬)伴随着动态的膜重构,如自噬体的形成以及自噬体与溶酶体的融合。然而,这种膜重塑的调节在很大程度上是未知的。由于我已经确定了三个Rab-GAP,Rab-type小GTP酶的灭活剂,作为自噬体驻留蛋白,在这里我解决了这些Rab-GAP(OATL 1/TBC 1D 25,TBC 1D 2B,TBC 1D 11)在自噬中的功能。它们定位于自噬体并与LC 3结合,LC 3是一种用作自噬体标记的自噬体驻留蛋白。TBC 1D 2B和TBC 1D 25需要与LC 3相互作用才能在自噬体中定位,因为取消相互作用的点突变会损害它们的定位。虽然我以前发现TBC 1D 25的过表达抑制了自噬体和溶酶体之间的融合,但TBC 1D 2B和TBC 1D 11的过表达并没有抑制自噬的任何过程。然而,我认为LC 3可能作为Rab-GAP的支架,除了在自噬中之外,还在膜运输中发挥作用。
英文摘要
Macroautpohagy (referred to as autophagy hereafter) accompanies dynamic membrane remodeling such as autophagosome formation and fusion between autophagosome and lysosome. However, the regulation of such membrane remodeling is largely unknown. Since I had identified three Rab-GAPs, inactivators of Rab-type small GTPases, as autophagosome resident proteins, here I addressed the function of these Rab-GAPs (OATL1/TBC1D25, TBC1D2B, TBC1D11) in autophagy. They were localized at autophagosomes and bound with LC3, an autophagosome resident protein used as a marker of autophagosome. TBC1D2B and TBC1D25 required the interaction with LC3 for their localization at autophagosomes, since a point mutation that abolished the interaction impaired their localization. Although I previously revealed that overexpression of TBC1D25 inhibits fusion between autophagosomes and lysosomes, overexpression of TBC1D2B nor TBC1D11 did not inhibit any process of autophagy.These results so far did not clarify relationship between these Rab-GAPs and autophagy. However, I suppose that it is possible that LC3 acts as a scaffold for Rab-GAPs and has a role in membrane trafficking in addition to in autophagy.
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会议论文
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [Itoh, T., Fujita, N., Saitoh, T., Komatsu, M, Akira, S., Yoshimori, T., and Fukuda, M., 伊藤敬]
通讯作者: 伊藤敬
Functional Analysis of Rab33B in Autophagy
Rab33B 在自噬中的功能分析
DOI: --
发表时间: 2011
期刊:
影响因子: --
作者: [Itoh, T.]
通讯作者: T.
DOI: 10.1111/j.1600-0854.2011.01240.x
发表时间: 2011-10-01
期刊: TRAFFIC
影响因子: 4.5
作者: [Matsui, Takahide, Itoh, Takashi, Fukuda, Mitsunori]
通讯作者: Fukuda, Mitsunori
A possible regulation of Rab33B-dependent membrane trafficking by the Atg12-5/16L1 complex
Atg12-5/16L1 复合物对 Rab33B 依赖性膜运输的可能调节
DOI: --
发表时间: 2012
期刊:
影响因子: --
作者: [Itoh, T., Fujita, N., Saitoh, T., Komatsu, M, Akira, S., Yoshimori, T., and Fukuda, M.]
通讯作者: M.
Development of defect evaluation method for photoabsorption layer in compound thin film solar cells and evaluation using it
  • 批准号:
    17K06345
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $3.0万
  • 财政年份:
    2017
  • 负责人:
    ITOH Takashi
  • 依托单位:
In Situ Raman Spectroelectrochemistry for Solvated Molecules at High Energy Interface
  • 批准号:
    15H03847
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 资助金额:
    $11.23万
  • 财政年份:
    2015
  • 负责人:
    ITOH Takashi
  • 依托单位:
The transformation of the world petroleum industry and business activities of major petroleum companies since the early 1980s
  • 批准号:
    15K03567
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $2.91万
  • 财政年份:
    2015
  • 负责人:
    ITOH Takashi
  • 依托单位:
Frontier Research Institute for Interdisciplinary Sciences
  • 批准号:
    25620146
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
  • 资助金额:
    $2.58万
  • 财政年份:
    2013
  • 负责人:
    ITOH Takashi
  • 依托单位:
海外基金