Search for novel membrane trafficking pathway involved in autophagy
Search for novel membrane trafficking pathway involved in autophagy
批准号:
23770216
负责人:
ITOH Takashi
金额:
$2.91万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Young Scientists (B)
财政年份:
2011
资助国家:
日本
项目状态:
已结题
起止时间:
2011 至 2012
中文摘要
巨自噬(以下简称自噬)伴随着动态的膜重塑,如自噬小体的形成和自噬小体与溶酶体间的融合。然而,这种膜重塑的调节在很大程度上是未知的。由于我已经确定了三个Rab-Gap,即Rab型小GTP酶的失活者,作为自噬小体驻留蛋白,所以在这里我讨论了这些Rab-Gap(OATL1/TBC1D25,TBC1D2B,TBC1D11)在自噬中的功能。它们定位于自噬小体,并与自噬小体驻留蛋白Lc3结合,作为自噬小体的标志。TbC1D2B和TbC1D25需要与Lc3相互作用才能定位在自噬小体上,因为取消相互作用的点突变削弱了它们的定位。虽然我之前发现过表达TBC1D25抑制自噬小体和溶酶体之间的融合,但过表达TBC1D2B和TBC1D11并不抑制任何自噬过程。这些结果到目前为止还没有阐明这些Rab-Gap和自噬之间的关系。然而,我认为Lc3可能作为Rab-Gap的支架,除了在自噬中发挥作用外,还在膜运输中发挥作用。
英文摘要
Macroautpohagy (referred to as autophagy hereafter) accompanies dynamic membrane remodeling such as autophagosome formation and fusion between autophagosome and lysosome. However, the regulation of such membrane remodeling is largely unknown. Since I had identified three Rab-GAPs, inactivators of Rab-type small GTPases, as autophagosome resident proteins, here I addressed the function of these Rab-GAPs (OATL1/TBC1D25, TBC1D2B, TBC1D11) in autophagy. They were localized at autophagosomes and bound with LC3, an autophagosome resident protein used as a marker of autophagosome. TBC1D2B and TBC1D25 required the interaction with LC3 for their localization at autophagosomes, since a point mutation that abolished the interaction impaired their localization. Although I previously revealed that overexpression of TBC1D25 inhibits fusion between autophagosomes and lysosomes, overexpression of TBC1D2B nor TBC1D11 did not inhibit any process of autophagy.These results so far did not clarify relationship between these Rab-GAPs and autophagy. However, I suppose that it is possible that LC3 acts as a scaffold for Rab-GAPs and has a role in membrane trafficking in addition to in autophagy.
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オートファジーに関わるRab不活性化因子の探索
寻找参与自噬的Rab失活因子
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Itoh, T., Fujita, N., Saitoh, T., Komatsu, M, Akira, S., Yoshimori, T., and Fukuda, M., 伊藤敬]
通讯作者:
伊藤敬
Functional Analysis of Rab33B in Autophagy
Rab33B 在自噬中的功能分析
DOI:
--
发表时间:
2011
期刊:
影响因子:
--
作者:
[Itoh, T.]
通讯作者:
T.
A possible regulation of Rab33B-dependent membrane trafficking by the Atg12-5/16L1 complex
Atg12-5/16L1 复合物对 Rab33B 依赖性膜运输的可能调节
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Itoh, T., Fujita, N., Saitoh, T., Komatsu, M, Akira, S., Yoshimori, T., and Fukuda, M.]
通讯作者:
M.
A possible regulation of Rab33B-dependent membrane trafficking by the Atg12-5/16L1 complex.
Atg12-5/16L1 复合物对 Rab33B 依赖性膜运输的可能调节。
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Itoh, T., Fujita, N., Saitoh, T., Komatsu, M, Akira, S., Yoshimori, T., and Fukuda, M.]
通讯作者:
M.
DOI:
10.1111/j.1600-0854.2011.01240.x
发表时间:
2011-10-01
期刊:
TRAFFIC
影响因子:
4.5
作者:
[Matsui, Takahide, Itoh, Takashi, Fukuda, Mitsunori]
通讯作者:
Fukuda, Mitsunori
Development of defect evaluation method for photoabsorption layer in compound thin film solar cells and evaluation using it
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批准号:17K06345
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.0万
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财政年份:2017
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负责人:ITOH Takashi
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依托单位:
In Situ Raman Spectroelectrochemistry for Solvated Molecules at High Energy Interface
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批准号:15H03847
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项目类别:Grant-in-Aid for Scientific Research (B)
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The transformation of the world petroleum industry and business activities of major petroleum companies since the early 1980s
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批准号:15K03567
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.91万
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财政年份:2015
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Frontier Research Institute for Interdisciplinary Sciences
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批准号:25620146
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项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.58万
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财政年份:2013
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依托单位:
The development of antidiabetic and antithrombogenic drugs using marine natural products as lead compounds
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批准号:24590145
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.41万
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财政年份:2012
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依托单位:
Functional analysis of Rab33B in autophagy.
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批准号:21770198
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.91万
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财政年份:2009
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负责人:ITOH Takashi
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依托单位:
A historical inquiry into the reorganization of business activities carried out by international petroleum companies in the 1970s.
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批准号:21530328
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.75万
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财政年份:2009
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负责人:ITOH Takashi
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依托单位:
Synthetic Studies of Isoquinoline and Indole Alkaloids Using Asymmetric Catalysis
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批准号:20590012
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$3.08万
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财政年份:2008
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负责人:ITOH Takashi
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依托单位:
Relation between Technological Museum Image and Curator Education in the New Age
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批准号:20605009
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.83万
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财政年份:2008
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负责人:ITOH Takashi
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依托单位:
Embedding of ordered normed spaces into numerical radius operator spaces
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批准号:20540156
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.25万
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财政年份:2008
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负责人:ITOH Takashi
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依托单位:
Analysis of regulation of Rab27A responsible for human inheritance disease Griscelli syndrome.
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批准号:19790242
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项目类别:Grant-in-Aid for Young Scientists (B)
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资助金额:$2.42万
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财政年份:2007
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负责人:ITOH Takashi
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依托单位:
Oxide Chemistry for in-situ Electrochemical Raman Spectroscopy
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批准号:18685021
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项目类别:Grant-in-Aid for Young Scientists (A)
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资助金额:$19.05万
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财政年份:2006
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负责人:ITOH Takashi
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依托单位:
A Study of Embeddings of Normed Spaces into Numerical Radius Operator Spaces
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批准号:18540159
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$0.65万
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财政年份:2006
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负责人:ITOH Takashi
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依托单位:
Total Synthesis of Alkaloids usingAsymmetric Catalysts
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批准号:18590016
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.57万
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财政年份:2006
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负责人:ITOH Takashi
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依托单位:
The Theoretical and Practical Study on the Oral History Method in the Historical Studies of Civil Engineering by Focusing the Expressway
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批准号:14350279
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$7.74万
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财政年份:2002
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负责人:ITOH Takashi
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依托单位:
The Research on the Present Stage of the World Petroleum Industry
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批准号:14530085
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.98万
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财政年份:2002
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负责人:ITOH Takashi
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依托单位:
STUDY ON PHASE TRANSITION AND CRITICAL PHENOMENA IN POLYMER CRYSTALS BASED ON LIGHT-HEATING MODULATED DTA AND HIGH PRESSURE MEASUREMENT
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批准号:14340122
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$8.77万
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财政年份:2002
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负责人:ITOH Takashi
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Construction and estimation of a novel isolation method for thermophilic archaea from hot springs.
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批准号:13660338
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$0.83万
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财政年份:2001
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负责人:ITOH Takashi
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依托单位:
STUDY ON CRITICAL PHENOMENA IN POLYMER CRYSTALS BASED ON LIGHT-HEATING MODULATED TEMPERATURE DIFFERENTIAL SCANNING CALORIMETRY UNDER HIGH PRESSURE
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批准号:12650885
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$2.37万
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财政年份:2000
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负责人:ITOH Takashi
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依托单位:
Organic Chemistry of Nitric Oxide for the Understanding of Its Physiological Roles
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批准号:10671996
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项目类别:Grant-in-Aid for Scientific Research (C)
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资助金额:$1.66万
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财政年份:1998
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负责人:ITOH Takashi
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依托单位:
海外基金