The role of phosphorylation status of radixin on the localization of billiary transporters

点击翻译按钮获取中文摘要

In this study, we examined localization of biliary transporters and physiological liver failure in chronic hepatitis by using human liver slices. We could determine the new binding partners (AnexinA2 etc) of biliary transporters by using two-dimensional fluorescence differential analysis with Ethan DIGE. And we could characterize AnexinA2 which negatively regulates the canalicular membrane localization of Mrp2 and Bsep. Moreover, chlestatic drugs(CyclopsorinA and Glibenclamide), including inhibitors of Bsep, one of biliary transporter, shows hepatotoxicity in the presence of the concentrated bile acids mixtureby using human sandwich cultured hepatocytes.