Elucidation of the mechanism of VA-RNA-induced innate immune response

我们构建了VA-RNA-deleted Ad（Ad△VR）载体，其中VA-RNA表达的转录调控元件被删除。尽管常规HEK 293细胞不支持Ad△VR载体的增殖，但通过适当诱导VA-RNA I表达而诱导表达VA-RNA I的HEK 293转化体（VR 293细胞）允许Ad△VR载体的增殖。在体外培养的细胞中，Ad△VR载体的转导效率可与传统的FG Ad载体相媲美。Ad△VR载体可能是FG Ad载体的一种更安全的替代载体。

We have developed a VA-RNA-deleted Ad(Ad△VR)vector, in which the transcriptional control elements of the VA-RNA-expression were deleted. Although conventional HEK293 cells did not support the propagation of the Ad△VR vectors, HEK293 transformants inducibly expressing VA-RNA I (VR293 cells) with appropriate induction of VA-RNA I expression allowed the propagation of the Ad△VR vector. The Ad△VR vector showed high transduction efficiency comparable to that of the conventional FG-Ad vector in the cultured cells. The Ad△VR vector may be a safer alternative to the FG-Ad vector.