Discovery of novel autoantibodies of fulminant type 1diabetes and autoimmune pancreatitis, the establishment of a measurement system.

发现暴发性1型糖尿病和自身免疫性胰腺炎新型自身抗体，建立检测体系。

• 批准号: 23790620
• 负责人: TAKIZAWA Soichi
• 金额: $ 2.83万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790620/
• 关键词: 免疫血清学; 免疫学; 糖尿病

We screened the human pancreas cDNA library using serum of theautoimmune pancreatitis with diabetes. I reported the new autoantibody which were common to autoimmune pancreatitis and fulminant type 1 diabetes mellitus by a preliminary experiment. I built the Enzyme-linked immunosorbent assay (ELISA) for detecting autoantibody against human amylase. As a result of having triedidentification of the epitope of the amylase antibody, I was able to pinpoint the part that antigenicity was high in. I try to build the ELISA having high sensitivity and specificity more now.

我们用自身免疫性胰腺炎合并糖尿病患者的血清筛选人胰腺cDNA文库。本文报道了一种新的自身抗体，该抗体是自身免疫性胰腺炎和暴发性1型糖尿病的共同抗体。建立了检测抗人淀粉酶自身抗体的酶联免疫吸附试验（ELISA）。由于对淀粉酶抗体的表位进行了三重鉴定，我能够确定抗原性高的部分。本研究旨在建立高灵敏度、高特异性的ELISA方法。