The elucidation of the progeny of the Eppk1-expressing cells: insightsinto the pancreatic exocrine regeneration mechanism

阐明 Eppk1 表达细胞的后代：深入了解胰腺外分泌再生机制

• 批准号: 23790797
• 负责人: MIKI Rika
• 金额: $ 2.5万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790797/
• 关键词: 幹細胞; 外分泌細胞; β細胞; 糖尿病; 再生医学; 膵再生; 膵炎

Epiplakin (Eppk1) was one of the pancreatic stem cell markers during the development.We hypothesized that Eppk1-expressing cells are one source of regenerating cells.Eppk1-CreER; Z/EG mice were administrated with tamoxifen and then injected caerulein thatcauses acinar cell degeneration. GFP labeled cells were detected in the Eppk1 negativeacinar cells. These result indicated that Eppk1 expressing cells were one source of theregenerated acinar cells.

Eppk1是胰腺干细胞发育过程中的标志物之一。我们假设Eppk1表达的细胞是再生细胞的来源之一。Eppk1-Creer；Z/EG小鼠给予他莫昔芬，然后注射雨蛙素导致腺泡细胞变性。在Eppk1阴性的腺泡细胞中可见GFP标记细胞。这些结果表明，Eppk1表达的细胞是退化腺泡细胞的来源之一。

项目成果

Recovery from diabetes in neonatal mice after a low-dose streptozotocin treatment

• DOI: 10.1016/j.bbrc.2012.12.030
• 发表时间: 2013-01-18
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Kataoka, Masateru;Kawamuro, Yuki;Kume, Shoen
• 通讯作者: Kume, Shoen

Regeneration of beta cells in neonatal mouse after streptozotocin treatment

链脲佐菌素治疗后新生小鼠β细胞的再生

• 发表时间: 2010
• 作者: Kawamura Y;Miki R;Yasukawa T;Yoshida T;Shiraki N;Kume K;Kume S
• 通讯作者: Kume S

Stem CellBiology: Basic Concepts to FrontiersStudents Edition, Chapter 9.ENDODERM

干细胞生物学：前沿基本概念学生版，第 9 章。ENDODERM

• 发表时间: 2012
• 作者: Katsumoto K.;Miki R.;Umeda;K.,Shiraki;N.;Kume;S.
• 通讯作者: S.