Improvement of serological diagnosis of leptospirosis and its epidemiological dynamics

钩端螺旋体病血清学诊断的改进及其流行病学动态

• 批准号: 23791130
• 负责人: RIE ISOZUMI
• 金额: $ 2.66万
• 依托单位: Osaka City University (2012-2013)Hokkaido University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23791130/
• 关键词: レプトスピラ症; 血清学的診断; ELISA; 疫学動態; 熱帯地域; ELISA法; 早期診断

Leptospirosis is wide spread disease especially in tropical developing countries, which cause symptom in human often severe to lethal. Early diagnosis and early treatment is quite important to avoid bad outcome, but it is difficult to diagnose at early stage of disease by current diagnose methods whose sensitivity and specificity are also insufficient to do complete diagnosis. With the diagnostic problem, we do not understand accurate epidemic status of leptospirosis in tropical developing countries. In this study, we developed a new diagnostic method based on ELISA with recombinant antigens (LipL32 and LigA). We tested samples from epidemic area (Thailand, Sri Lanka and Vietnam) with this method. We also investigated wild rats in Hanoi which are important vector of leptospirosis.

钩端螺旋体病是一种广泛传播的疾病，特别是在热带发展中国家，它会导致人类症状严重甚至致死。早期诊断、早期治疗对避免不良结局十分重要，但目前的诊断方法很难做到早期诊断，其敏感性和特异性也不足以做出完整的诊断。由于诊断问题，我们无法了解热带发展中国家钩端螺旋体病的准确流行状况。在本研究中，我们建立了一种新的基于重组抗原(LipL32和LIGA)的ELISA诊断方法。我们用这种方法对疫区(泰国、斯里兰卡和越南)的样本进行了检测。我们还调查了河内的野生老鼠，它们是钩端螺旋体病的重要媒介。

项目成果

Characteristic Age Distribution of Plasmodium vivax Infections after Malaria Elimination on Aneityum Island, Vanuatu

• DOI: 10.1128/iai.00931-13
• 发表时间: 2014-01-01
• 期刊: INFECTION AND IMMUNITY
• 影响因子: 3.1
• 作者: Kaneko, Akira;Chaves, Luis F.;Drakeley, Chris
• 通讯作者: Drakeley, Chris

A survey of rodent-borne pathogens carried by wild Rattus spp. in Northern Vietnam

• DOI: 10.1017/s0950268812002385
• 发表时间: 2013-09-01
• 期刊: EPIDEMIOLOGY AND INFECTION
• 影响因子: 4.2
• 作者: Koma, T.;Yoshimatsu, K.;Arikawa, J.
• 通讯作者: Arikawa, J.

Application of Truncated Nucleocapsid Protein (N) for Serotyping ELISA of Murinae-Associated Hantavirus Infection in Rats

截短核衣壳蛋白 (N) 在大鼠鼠相关汉坦病毒感染血清分型 ELISA 中的应用

• 发表时间: 2012
• 期刊: J Vet Med Sci
• 影响因子: 1.2
• 作者: Yasuda SP;Yoshimatsu K;Koma T;Shimizu K;Endo R;Isozumi R;Arikawa J
• 通讯作者: Arikawa J

Plasmodium falciparum : differential selection of drug resistance alleles in contiguous urban and per -urban areas of Brazzaville, Republic of Congo

恶性疟原虫：刚果共和国布拉柴维尔邻近城市和城郊地区耐药等位基因的差异选择

• 发表时间: 2011
• 期刊: PLoS One
• 影响因子: 3.7
• 作者: Tsumori;Y.; Ndounga;M.; Sunahara;T.; Hayashida;N.; Inoue;M.; Nakazawa;S.; Casimiro;P.; Isozumi;R.; Uemura;H.; Tanabe;K.; Kaneko;O.; Culleton;R.
• 通讯作者: R.

スリランカ産Rattus rattusおよびBandicota bengalensisの系統学的位置

斯里兰卡黄毛鼠和孟加拉斑皮鼠的系统发育位置

• 发表时间: 2012
• 作者: 安田俊平;Chandika D. Gamage;小泉信夫;西尾佐奈恵;五十棲理恵;清水健太;駒貴明;天田貴子;鈴木仁;吉松組子;有川二郎
• 通讯作者: 有川二郎