Improvement of serological diagnosis of leptospirosis and its epidemiological dynamics

钩端螺旋体病是一种广泛传播的疾病，特别是在热带发展中国家，其引起的症状往往严重到致命。早期诊断和早期治疗对于避免不良后果非常重要，但现有的诊断方法难以在疾病早期诊断，其敏感性和特异性也不足以做出完整的诊断。由于诊断问题，我们不了解钩端螺旋体病在热带发展中国家的准确流行状况。在本研究中，我们建立了一种基于重组抗原（LipL32和LigA）的ELISA诊断方法。我们采用该方法对疫区（泰国、斯里兰卡和越南）的样品进行了检测。我们还调查了河内市钩端螺旋体病的重要媒介野生大鼠。

Leptospirosis is wide spread disease especially in tropical developing countries, which cause symptom in human often severe to lethal. Early diagnosis and early treatment is quite important to avoid bad outcome, but it is difficult to diagnose at early stage of disease by current diagnose methods whose sensitivity and specificity are also insufficient to do complete diagnosis. With the diagnostic problem, we do not understand accurate epidemic status of leptospirosis in tropical developing countries. In this study, we developed a new diagnostic method based on ELISA with recombinant antigens (LipL32 and LigA). We tested samples from epidemic area (Thailand, Sri Lanka and Vietnam) with this method. We also investigated wild rats in Hanoi which are important vector of leptospirosis.