Approach to reveal dynamics of caspase activation in maintainingbrain homeostasis

揭示半胱天冬酶激活维持脑稳态动态的方法

• 批准号: 23659032
• 负责人: YAMAGUCHI Yoshifumi
• 金额: $ 2.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659032/
• 关键词: 脳; カスパーゼ; 神経免疫; 睡眠

Caspases are evolutionary conserved cystein protease. In this study, we tried to reveal dynamics of caspase activation in maintaining brain homeostasis. For this purpose, we utilized two novel probes that allow us to detect caspases-1 or caspase-3 in real-time. However, we have not detected weak or mild activation of those caspases in the brain derived from sleep-deprived mouse or in primary cell culture. These results suggest that further improvement in detection method will be needed to observe weak or mild activation of caspases by these probes.

半胱天冬酶是进化上保守的半胱氨酸蛋白酶。本研究试图揭示半胱天冬酶在维持脑内稳态中的激活动力学。为此，我们利用了两种新的探针，使我们能够实时检测半胱天冬酶-1或半胱天冬酶-3。然而，我们没有检测到弱或轻度激活的那些半胱天冬酶在大脑中来自睡眠剥夺小鼠或在原代细胞培养。这些结果表明，需要进一步改进检测方法，以观察这些探针对半胱天冬酶的弱或轻度激活。

项目成果

Evaluation of SCAT1: a genetically- encoded probe for detecting activation of caspaes- 1 in living cells

SCAT1 的评估：用于检测活细胞中 Caspaes-1 激活的基因编码探针

• 发表时间: 2011
• 作者: Koichi Shikada;Yoshifumi Yamaguchi;Ting Liu;Kiwamu Takemoto;Katsuaki Hoshino;Tsuneyasu Kaisho;Erina Kuranaga;Masayuki Miura
• 通讯作者: Masayuki Miura

Live imaging of apoptosis in a novel transgenic mouse highlights its role in neural tube closure.

• DOI: 10.1083/jcb.201104057
• 发表时间: 2011-12-12
• 期刊: The Journal of cell biology
• 作者: Yamaguchi Y;Shinotsuka N;Nonomura K;Takemoto K;Kuida K;Yosida H;Miura M
• 通讯作者: Miura M

COLD SPRING HARBOR ASIA CONFERENCES

冷泉港亚洲会议

• 发表时间: 2013

Evaluation of SCAT1: a genetically-encoded probe for detecting activation of caspaes-1 in living cells

SCAT1 的评估：用于检测活细胞中 caspaes-1 激活的基因编码探针

• 发表时间: 2011
• 作者: Koichi Shikada;Yoshifumi Yamaguchi;Ting Liu;Kiwamu Takemoto;Katsuaki Hoshino;Tsuneyasu Kaisho;Erina Kuranaga;Masayuki Miura
• 通讯作者: Masayuki Miura

Visualization of Inflammasome Activation: Monitoring Caspase-1 Activation in Living Cells by a Genetically-encoded Probe SCAT1

炎症小体激活的可视化：通过基因编码探针 SCAT1 监测活细胞中的 Caspase-1 激活

• 作者: Koichi Shikada;Yoshifumi Yamaguchi;Ting Liu;Kiwamu Takemoto;Katsuaki Hoshino;Tsuneyasu Kaisho;Erina Kuranaga;Masayuki Miura;Ting Liu
• 通讯作者: Ting Liu