Analysis of the molecular mechanism of flip-flop that regulates the asymmetric distribution of lipids in the plasma membranes

调控质膜脂质不对称分布的触发器的分子机制分析

• 批准号: 23659047
• 负责人: HASEGAWA Akiko
• 金额: $ 2.41万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659047/
• 关键词: フリップ; フロップ; ホスファチジルセリン; 脂質動態; 形質膜; 非対称分布; 変異株; フリップ・フロップ

An adaptor protein was identified as the responsible factor of the mutant which was thought to have a defect of flip-flop machinery. The results of the RNAi experiment suggested the possibility that the adaptor protein contributes to the regulation of transbilayer movement of phosphatidylserine at the plasma membranes. I tried to identify the molecules which take part in the flip-flop machinery in cooperation with the adaptor protein. However, it has not succeeded yet. I also analyzed the signaling pathway involved in flip-flop machinery, and the relationship between flip-flop and cytoskeleton was implied.

一种接头蛋白被确定为突变的负责因素，该突变被认为具有触发器机制的缺陷。RNAi实验结果提示该接头蛋白可能参与了磷脂酰丝氨酸在质膜上的跨双分子层运动调控。我试着找出那些与接头蛋白合作参与这种翻转机制的分子。然而，它还没有成功。我还分析了触发器机制所涉及的信号通路，并暗示了触发器与细胞骨架之间的关系。

项目成果

細胞形質膜の内外層非対称性を制御するフリップ・フロップ機構

控制质膜内外层不对称性的触发器机制

• 发表时间: 2011
• 作者: Akiko Yamaji-Hasegawa;Akira Hattori;Masafumi Tsujimoto;Toshihide Kobayashi;山路顕子
• 通讯作者: 山路顕子

14-3-3 regulates the transbilayer movement of phosphatidylserine by altering actin assembly

14-3-3 通过改变肌动蛋白组装来调节磷脂酰丝氨酸的跨双层运动

• 发表时间: 2013
• 作者: Akiko Yamaji-Hasegawa;Akira Hattori;Masafumi Tsujimoto;Toshihide Kobayashi
• 通讯作者: Toshihide Kobayashi