Identification of the genes related to longevity and cancer resistance in Naked mole-rats.

裸鼹鼠长寿和抗癌相关基因的鉴定。

• 批准号: 23659163
• 负责人: TERADA Yasuhiko
• 金额: $ 2.41万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659163/
• 关键词: 抗老化遺伝子; 発現クローニング; 遺伝子ライブラリー; ハダカデバネズミ

Naked mole-rats (Hg:Heterocephalus glaber) are extremely long-lived (30 years) mouse-sized rodents. In addition to its longevity, naked mole-rats have an extraordinary resistance to cancer as tumors have never been observed in these rodents. However, the mechanisms responsible for them were unknown at all.In order to examine these mechanisms, we isolated several cDNAs responsible for the longevity and cancer resistance by expression cloning. For these purposes, we generated the retrovirus-based cDNA libraries from the livers of the Naked mole-rats, and infected MEF (mouse embryo fibroblasts) or v-ras-transformed NIH3T3 cells. Of several cDNAs, we found cDNA related to intermediate filament was linked to cancer resistance in the Naked mole-rats.

裸mole鼠（HG：Heterocephalus Glaber）是非常长的（30年）小鼠大小的啮齿动物。除了其寿命外，裸鼠鼠对癌症具有极大的抵抗力，因为在这些啮齿动物中从未观察到肿瘤。然而，为了检查这些机制而言，负责它们的机制根本是未知的，我们分离了几种通过表达克隆来负责寿命和癌症耐药性的cDNA。为了这些目的，我们从裸痣鼠的肝脏中生成了基于逆转录病毒的cDNA库，并感染了MEF（小鼠胚胎成纤维细胞）或V-RAS转换的NIH3T3细胞。在几种cDNA中，我们发现与中间细丝相关的cDNA与裸鼠鼠的癌症抗性有关。