A basic studyon malignant mesothelioma pathogenesis by internal radiation exposure of radium contained in the iron-containing protein

10只健康雄性SLC：Wistar大鼠多次注射(Ip)。用一种螯合剂和铁。10只大鼠中有5只出现恶性腹膜间皮瘤，而对照组和络合剂给药组均未见。肿瘤细胞Calretinin和CEA染色阳性。免疫组织化学染色结果与电子显微镜检查结果一致，提示为上皮性间皮瘤。此外，8-羟基-2-脱氧鸟苷免疫染色为DNA氧化损伤标志物阳性。于是，传统的理论得到了证实。目前正在对包括镭在内的微量元素进行最后分析。

Ten healthy male Slc: Wistar rats were repeatedly injected (i.p.) with a chelating agent and iron. Five of 10 rats showed a malignant peritoneal mesothelioma, but no rats showed it in the control and the chelating agent administration group. The tumor cells were positively stained by Calretinin and CEA. The results of immunostaining and the findings by electron microscope indicated epithelial mesothelioma. In addition, immunostaining with 8-hydroxy-2-deoxyguanosine is a DNA oxidative damage marker was positive. Thus, the conventional theoriy was confirmed. The final analysis of trace elements including radium is ongoing.