Establishment of therapeutic assessment in glaucoma neuroprotection using live-imaging of axonal transport in optic nerve

使用双光子显微镜对荧光蛋白在神经元线粒体中特异性表达的小鼠进行体内成像。采用激光照射小鼠巩膜静脉建立高眼压模型。视网膜神经节细胞的活体成像显示，在小鼠青光眼模型中，RGC死亡前线粒体轴突运输紊乱，提示异常轴突运输参与青光眼病理

In vivo imaging of the mice in which fluorescent proteins areexpressed specifically in neuronal mitochondria was conducted using two-photonmicroscopy. As a glaucoma model, ocular hypertension was induced by laser treatment ofepiscleral veins in mice. In vivo imaging of retinal ganglion cells reveals thedisturbance of mitochondrial axonal transport in the mouse glaucoma model before RGC death,suggesting that the abnormal axonal transport is involved in glaucoma pathology