Establishment of assay systems for risk assessment of exposure to endocrine disrupting chemicals in human developing brain

本研究旨在建立一种有效的实验动物内分泌干扰物暴露风险评估系统。在本试验系统中，引入大鼠（褐家鼠）和食蟹猴（食蟹猴）作为有用的实验动物，以评价啮齿动物和灵长类动物对特定化学品的反应之间的任何种属差异。此外，本研究结合体外（使用活体动物）和体内（使用原代培养细胞）研究，试图产生关于化学品暴露不良反应的任何生物学可靠和准确的信息。这项研究表明，猴子和大鼠对甲状腺激素缺乏和接触合成化合物的反应存在显著的物种差异。此外，体内和体外研究的结合成功地产生了关于测试化合物效果的生物学可靠信息。

This study aimed to establish an effective assay system using experimental animals for the risk assessment of exposure to endocrine disrupting chemicals in developing human. In the present assay system, rats (Rattus norvegicus) and cynomolgus monkeys (Macaca fascicularis) were introduced as useful experimental animals to evaluate any species difference between rodent and primate in the responses to a specific chemical. In addition, this study tried to produce any biologically reliable and accurate information about the adverse effects of chemical exposure, combining in vitro (using living animals) and in vivo (using primary cultured cells) study. This study revealed that there were remarkable species differences between monkeys and rats in the response to thyroid hormone deficiency and exposure to synthetic compounds. Furthermore, the combination of in vivo and in vitro studies succeeded in producing the biologically reliable information about the effects of the compound tested.