Analysis of amino acid signaling in mammals and Drosophila

哺乳动物和果蝇的氨基酸信号传导分析

• 批准号: 23687031
• 负责人: NISHIMURA Takashi
• 金额: $ 15.39万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23687031/
• 关键词: シグナル伝達; アミノ酸; 個体成長; インスリン様成長因子; ショウジョウバエ; 生理学; 発生・分化; 遺伝学; 蛋白質; アミノ酸シグナル; 成長制御

The multi-protein complex TORC1 is a key regulator of cell growth and size control in both Drosophila and mammals. To better understand amino acid signaling, we took a biochemical approach to identify the binding proteins of small GTPase RagA/C. We identified the glycolytic enzyme GAPDH and found that GAPDH likely regulates the TORC1 localization and activity through RagA/C.In addition, to understand how Drosophila recognizes nutrition to control body growth, we focused on the molecular mechanism underlying the nutrient-dependent expression of Drosophila Insulin-like peptide (dilp). We identified the responsible transcription factors that cooperatively control dilp5 gene expression. We also conducted in vivo RNAi screening to identify novel players for the regulation of body growth. We identified a gene that we named SDR. SDR is a new class of secreted Dilp-binding proteins that negatively regulate the function of Dilps and thereby body growth.

多蛋白复合物 TORC1 是果蝇和哺乳动物细胞生长和大小控制的关键调节因子。为了更好地理解氨基酸信号传导，我们采用生化方法来鉴定小 GTPase RagA/C 的结合蛋白。我们鉴定了糖酵解酶GAPDH，并发现GAPDH可能通过RagA/C调节TORC1的定位和活性。此外，为了了解果蝇如何识别营养来控制身体生长，我们重点关注果蝇胰岛素样肽（dilp）营养依赖性表达的分子机制。我们确定了协同控制 dilp5 基因表达的负责转录因子。我们还进行了体内 RNAi 筛选，以确定调节身体生长的新参与者。我们鉴定了一个基因，并将其命名为 SDR。 SDR 是一类新型的分泌型 Dilp 结合蛋白，可负向调节 Dilps 的功能，从而调节身体生长。

项目成果

Regulatory mechanism of the Drosophila insulin-like peptide gene expression in insulin-producing cells

果蝇胰岛素样肽基因在胰岛素产生细胞中表达的调控机制

• 发表时间: 2012
• 作者: 岡本直樹;西村隆史
• 通讯作者: 西村隆史

A transgenic RNA interference screen for regulators of nutrient-dependent growth in the fruit fly Drosophila melanogaster

果蝇营养依赖性生长调节因子的转基因 RNA 干扰筛选

• 发表时间: 2011
• 作者: Naoki Okamoto;Takashi Nishimura
• 通讯作者: Takashi Nishimura

体の成長を制御するホルモンの新受容体を発見

发现控制身体生长的激素新受体

Regulatory mechanism of the Drosophila insulin-like peptide gene expression

果蝇胰岛素样肽基因表达的调控机制

• 发表时间: 2012
• 作者: Naoki Okamoto;Takashi Nishimura
• 通讯作者: Takashi Nishimura

Regulation of the systemic growth by insulin-like peptides during Drosophila development

果蝇发育过程中胰岛素样肽对全身生长的调节

• 发表时间: 2012
• 作者: Naoki Okamoto;Tomoka Murai;Takashi Nishimura
• 通讯作者: Takashi Nishimura