Analysis of amino acid signaling in mammals and Drosophila

多蛋白复合物TORC1是果蝇和哺乳动物细胞生长和大小控制的关键调节因子。为了更好地理解氨基酸信号，我们采用生化方法鉴定了小GTPase RagA/C的结合蛋白。我们鉴定了糖酵解酶GAPDH，发现GAPDH可能通过RagA/C调控TORC1的定位和活性。此外，为了了解果蝇如何识别营养来控制身体生长，我们重点研究了果蝇胰岛素样肽（dilp）营养依赖性表达的分子机制。我们确定了共同控制dilp5基因表达的相关转录因子。我们还进行了体内RNAi筛选，以确定调节身体生长的新参与者。我们发现了一个基因，我们将其命名为SDR。SDR是一类新的分泌型dilp结合蛋白，可负性调节dilp的功能，从而调节机体的生长。

The multi-protein complex TORC1 is a key regulator of cell growth and size control in both Drosophila and mammals. To better understand amino acid signaling, we took a biochemical approach to identify the binding proteins of small GTPase RagA/C. We identified the glycolytic enzyme GAPDH and found that GAPDH likely regulates the TORC1 localization and activity through RagA/C.In addition, to understand how Drosophila recognizes nutrition to control body growth, we focused on the molecular mechanism underlying the nutrient-dependent expression of Drosophila Insulin-like peptide (dilp). We identified the responsible transcription factors that cooperatively control dilp5 gene expression. We also conducted in vivo RNAi screening to identify novel players for the regulation of body growth. We identified a gene that we named SDR. SDR is a new class of secreted Dilp-binding proteins that negatively regulate the function of Dilps and thereby body growth.