The effects of food protein-derived metabolite, indoxyl sulfate, on progression of renal disease.

食物蛋白衍生代谢物硫酸吲哚酚对肾脏疾病进展的影响。

• 批准号: 23700830
• 负责人: SHIMIZU Hidehisa
• 金额: $ 2.83万
• 依托单位: Hokkaido University (2012)Nagoya University (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23700830/
• 关键词: 慢性腎不全; 尿毒症物質; トリプトファン代謝物; インドキシル硫酸; 細胞老化; 線維化; 炎症反応; 繊維化

In patients with chronic kidney disease (CKD), serum indoxyl sulfate is elevated. Therefore, the present study examined what molecules are activated and expressed by indoxyl sulfate, and how indoxyl sulfate promotes CKD. Indoxyl sulfate activated ERK, p38, JNK, NF-?B, p53, Stat3, and CREB in proximal tubular cells. In addition, the expression of NOX4, TGF-?1, Smad3, ?-SMA, MCP-1, ICAM-1, and angiotensinogen was upregulated. Current study demonstrated that indoxyl sulfate induced macrophage infiltration, cellular senescence, and fibrosis through activation and expression of above-mentioned factors in process of CKD progression.

在慢性肾脏疾病(CKD)患者中，血清吲哚硫酸盐水平升高。因此，本研究考察了吲哚硫酸盐激活和表达哪些分子，以及吲哚硫酸盐如何促进CKD。硫代吲哚可激活近端肾小管上皮细胞ERK、p38、JNK、NF-βB、P53、STAT3和CREB。此外，NOX4、TGF-β1、Smad3、β-SMA、MCP-1、ICAM-1和血管紧张素原的表达上调。目前的研究表明，硫酸吲哚可通过激活和表达上述因子在CKD进展过程中诱导巨噬细胞的浸润、细胞衰老和纤维化。

项目成果

Indoxyl sulfate upregulates renal expression of MCP-1 via production of ROS and activation of NF-κB, p53, ERK, and JNK in proximal tubular cells

• DOI: 10.1016/j.lfs.2012.01.013
• 发表时间: 2012-04-09
• 期刊: LIFE SCIENCES
• 影响因子: 6.1
• 作者: Shimizu, Hidehisa;Bolati, Dilinaer;Niwa, Toshimitsu
• 通讯作者: Niwa, Toshimitsu

Accumulation and biological effects of metals in wild rats in mining areas of Zambia

• DOI: 10.1007/s10661-012-2912-6
• 发表时间: 2013-06-01
• 期刊: ENVIRONMENTAL MONITORING AND ASSESSMENT
• 影响因子: 3
• 作者: Nakayama, Shouta M. M.;Ikenaka, Yoshinori;Ishizuka, Mayumi
• 通讯作者: Ishizuka, Mayumi

Cytochrome P450 3A mRNA expression along goat and rat gastrointestinal tracts

山羊和大鼠胃肠道细胞色素 P450 3A mRNA 表达

• 发表时间: 2012
• 期刊: Jpn. J. Vet. Res.
• 作者: Dominic MUREITHI;Wageh Sobhy DARWISH;Yoshinori IKENAKA;Laetitia KANJA;Mayumi ISHIZUKA.
• 通讯作者: Mayumi ISHIZUKA.

Stat3 is involved in indoxyl sulfate-induced inflammatory and fibrotic gene expression and cellular senescence in proximal tubular cells.

Stat3 参与硫酸吲哚酚诱导的炎症和纤维化基因表达以及近端肾小管细胞的细胞衰老。

• 发表时间: 2012
• 作者: Niwa;T.;Nishijima;F.;Shimizu;H.
• 通讯作者: H.

Metal and metalloid levels and bio-accumulation characteristics in soil, sediment, land plants and hippopotami (Hippopotamus amphibius L) from the South Luangwa National Park, Zambia

赞比亚南卢安瓜国家公园土壤、沉积物、陆地植物和河马 (Hippopotamus amphibius L) 中的金属和类金属水平以及生物富集特征

• DOI: 10.1016/j.ecoenv.2012.04.003
• 发表时间: 2012
• 期刊: Ecotoxicology and Environmental Safety
• 影响因子: 6.8
• 作者: 中野伸彦;ほか;竹門康弘;市岡孝朗;中山翔太
• 通讯作者: 中山翔太