Roles of laminin g2 chain in tumor growth and invasion.

为了分析lmg2链单体在体内对肿瘤生长和侵袭的影响，我们在人膀胱癌细胞系EJ-1和人胚胎肾细胞系HEK-293中过表达lmg2链。在裸鼠皮下或腹腔注射时，与对照转染相比，γ - 2过表达细胞生长更快，产生更大的实体瘤。这些数据有力地表明，g2单体有助于肿瘤细胞在基质组织中的侵袭性生长。接下来，我们在HEK-293中过表达了三种不同的2片段，并分析了2链的活性位点。发现2链结构域蛋白V （domein V of 2 chain, 2dV）强烈诱导肿瘤生长。然后，我们建立了针对人层粘连蛋白2链V结构域的单克隆抗体。抗体的进一步详细表征正在进行中。

To analyze the effect of Lm g2 chain monomer on tumor growth and invasion in vivo, we overexpressed of the g2 chain in the human bladder carcinoma cell line EJ-1 and human embryonic kidney cell line HEK-293. When implanted into nude mice subcutaneously or intraperitoneally, gamma2 overexpressed cells grew faster and produced much larger solid tumors than the control transfectant. These data strongly suggest that the g2 monomer contribute to the invasive growth of tumor cells in stromal tissues. Next, we overexpressed three different 2 flagments in the HEK-293 and analyzed the active site of the 2 chain. It was found that domein V of 2 chain (2dV) strongly induced the tumor growth. Then, we established monoclonal antibodies against the domain V of human laminin 2 chain. Further detailed characterization of the antibody is in progress.