Search for antitumor compounds from symbiotic or parasitic microbes in aquatic multicellular organisms

本研究的目的是有效地寻找新的抗肿瘤药物。从水生生物中分离并培养了约1000株微生物。从代谢产物中分离出对癌细胞具有细胞毒性的化合物。鉴定了13个已知化合物，其中8个为新化合物。本研究建立了多细胞系细胞毒性的评价和比较体系。利用该系统，发现了一种新的化合物海藻糖，并推出了一种蛋白磷酸酶2A特异性抑制剂红曲霉毒素A作为分子探针。

In this study, the researcher aimed for efficient searching of new antitumor agents. About 1000 strains of microbes were isolated from aquatic organisms and cultured. Cytotoxic compounds against cancer cells were isolated from the metabolites. While 13 known compounds were identified, 8 compounds were estimated as novel compounds. The researcher established the evaluation and comparison system of cytotoxicity employing multiple culture cell lines. Harnessing the system, a novel compound lentztrehalose was discovered and a protein phosphatase 2A specific inhibitor rubratoxin A was launched as a molecular probe.