Microbial interaction of fluid and mineral phases at hydrothermal vents of the Mid-Atlantic Ridge

...该提案的主要目标是：分析流体，沉积物中存在的微生物群落和附着在矿物相及其时空变化取决于16S rDNA靶向DGGE细菌和微生物。热液、沉积物和矿物表面关键种群的鉴定及其荧光原位杂交检测。基于aprA基因片段的功能基因分析对硫酸盐还原和硫氧化微生物的检测和分布。通过基于培养的方法定量、富集和分离化能自养需氧和硝酸盐还原硫和氢氧化微生物。通过基于培养的方法定量、富集和分离自养硫酸盐还原微生物。富集和分离微生物，使其在可能催化“海洋沥滤”的矿物硫化物上生长。基于培养的方法和不同分子工具的组合将允许对所获得的结果进行验证。

... The mayor goals of this proposal are: Analysis of the microbial community present in the fluid, sediment and attached to mineral phases and their temporal and spatial variation depending on 16S rDNA targeted DGGE for Bacteria and Archaea. Identification of key population and their detection by fluorescence in situ hybridization in the hydrotheram fluid, sediment and on mineral surfaces. Detection and distribution of sulfate-reducing and sulfur-oxidizing microorganisms by functional gene analysis based on an aprA gene fragment. Quantification, enrichment, and isolation of chemolithoautotrophic aerobic and nitrate reducing sulfur- and hydrogen-oxidizing microorganisms by cultivation based methods. Quantification, enrichment, and isolation of autotrophic sulfate-reducing microorganisms by cultivation based methods. Enrichment and isolation of microorganisms to grow on mineral sulfides which might catalyze "marine leaching". The combination of cultivation based methods and differnt molecular tools will allow a verification of the obtained results.