Microbial interaction of fluid and mineral phases at hydrothermal vents of the Mid-Atlantic Ridge

..。这项提议的主要目标是：分析流体、沉积物和附着在矿物相上的微生物群落，以及它们的时空变化，这取决于16S rDNA针对细菌和古生物的DGGE。热液流体、沉积物和矿物表面关键种群的鉴定及其荧光原位杂交检测。基于APRA基因片段的功能基因分析检测硫酸盐还原和硫氧化微生物的分布以培养为基础的方法对化学自养好氧和硝酸盐还原硫氧化和氢氧化微生物的量化、浓缩和分离。用基于培养的方法对自养硫酸盐还原微生物进行量化、浓缩和分离。在可能催化“海洋淋洗”的矿物硫化物上生长的微生物的浓缩和分离。基于培养的方法和不同的分子工具的结合将允许对所获得的结果进行验证。

... The mayor goals of this proposal are: Analysis of the microbial community present in the fluid, sediment and attached to mineral phases and their temporal and spatial variation depending on 16S rDNA targeted DGGE for Bacteria and Archaea. Identification of key population and their detection by fluorescence in situ hybridization in the hydrotheram fluid, sediment and on mineral surfaces. Detection and distribution of sulfate-reducing and sulfur-oxidizing microorganisms by functional gene analysis based on an aprA gene fragment. Quantification, enrichment, and isolation of chemolithoautotrophic aerobic and nitrate reducing sulfur- and hydrogen-oxidizing microorganisms by cultivation based methods. Quantification, enrichment, and isolation of autotrophic sulfate-reducing microorganisms by cultivation based methods. Enrichment and isolation of microorganisms to grow on mineral sulfides which might catalyze "marine leaching". The combination of cultivation based methods and differnt molecular tools will allow a verification of the obtained results.