Analysis of EVI1-expressing leukemia using inv(3)(q21;q26) model mice

3q21和3q26之间的染色体易位和反转导致3q26上EVI1基因的错误表达，从而诱导白血病发生，诊断不佳。为了研究白血病发生的潜在机制，我们通过连接两个细菌人工染色体（BAC）克隆，建立了一种新的表达evi1的白血病小鼠模型（3q21q26小鼠），该模型含有一个反转等位基因的转基因。3q21q26小鼠在24周龄后出现髓系和淋巴系白血病。我们在3q21q26小鼠的骨髓中发现了异常的cd34高细胞部分，这表明这些细胞与白血病有关。

Chromosomal translocation and inversion between 3q21 and 3q26 result in misexpression of EVI1 gene on 3q26, which induces leukemogenesis with poor diagnosis. To examine the underlying mechanism of leukemogenesis, we generated a novel mouse model of EVI1-expressing leukemia (3q21q26 mice) harboring a transgene recapitulating the inverted allele by linking two bacterial artificial chromosome (BAC) clones. The 3q21q26 mice suffer from myeloid and lymphoid leukemia after 24 weeks of age. We discovered an abnormal CD34-high cell fraction specifically in the bone marrow of 3q21q26 mice, suggesting that these cells are associated with leukemia.