Basic study for the inhibitory effects of trehalose on malignant melanoma cells

将人皮肤恶性黑色素瘤细胞植入裸鼠结膜下间隙。在体内系统中灌注不同浓度的海藻糖，在不同浓度的海藻糖下培养黑色素瘤细胞，用MTT法分析肿瘤细胞增殖活性的变化，并用流式细胞术定量检测对细胞周期有重要影响的几种转录因子的表达（体外系统）。海藻糖具有剂量依赖性，通过转录因子抑制恶性细胞增殖，并与转录因子相关，导致肿瘤细胞凋亡。

Human cutaneous malignant melanoma cells were implanted in the subconjunctival spaces of nude rats. Various concentrations of trehalose were instilled (in vivo system) and melanoma cells were cultivated under various concentrations of trehalose for analyzing changes of proliferative activities of tumor cells by MTT assay and flow-cytometrically quantifying expressions of some kinds of transcription factors that were known to be important for cell cycle (in vitro system). Trehalose dose-dependently showed inhibition of malignant cell proliferation and through transcription factors and demonstrated to lead tumor cells to apoptosis in association with transcription factors.