Development of research methods for dry eye using stem cells

DryEye的治疗选择正在扩大。但是干眼症的评价体系还很小。本研究的目的是利用角膜上皮干细胞构建干眼评价系统。干细胞菌落可以在DMEM-F12、B-27、EGF存在的情况下取，它们能够证实粘蛋白的表达是pas阳性染色。使用FR1800204为ERK1、2抑制剂，有可能抑制粘蛋白的表达。此外，抑制ERK5除抑制ERK1.2外，还有可能抑制细胞生长。只有抑制ERK1, 2表明它不会对细胞生长产生不利影响。以获得可预期的结果作为新的评价体系模型。

Treatment options for DryEye is expanding. But the evaluation system for dry eye is small. The studies were carried out for the purpose of construction of dry eye evaluation system using the corneal epithelial stem cells in this study. Stem cell colonies can be taken in DMEM-F12, B-27, EGF presence, they were able to confirm expression of mucin is a PAS-positive staining. The use of FR1800204 is ERK1, 2 inhibitors for, it is possible to suppress the expression of mucin. In addition, inhibition of ERK5 in addition to the ERK1.2, it is possible to suppress cell growth. Only inhibition of ERK1, 2 revealed that it does not adversely affect cell growth. To obtain results that can be expected as a new evaluation system model.