X-ray crystallography of quasi-racemic glycoprotein synthesized by chemical methods

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In this study, a new peptide-thioester synthetic method and a regioselective peptide coupling reaction using peptide-guanidide, that was originally found by the author, have been successfully established for the synthesis of homogeneous glycoproteins. Based on these new methodologies, the syntheses of N-glycoprotein: CD59 and O-linked glycoprotein: anti freeze glycoprotein (AFGP) were also achieved. Furthermore, a diastereomeric form of AFGP which has D-amino acids and native GalNAc residues have also successfully synthesized. Quasi-racemic crystallization of AFGP was conducted by using the solution of the 1:1 mixture of the native AFGP and the diastereomeric AFGP.