Studies on the controlled degradation of polySia scaffolds

在WP6中，将制定能够诱导和控制PolySia支架降解的策略。聚唾液酸可以被噬菌体携带的酶--内唾液酸酶特异性地降解。这项研究的目的是利用分子生物学技术来调节内唾液酸酶的切割特性，并开发允许定点靶向和失活酶的策略。仿生矿化技术将被应用于提供具有更好的化学稳定性和降低对降解酶的敏感性的材料。某些矿物相，例如氧化铁或氢氧化物，可以为支架的特定位置提供额外的线索。WP2和WP3中研究的有机和聚合物化学方面以及WP4和WP5中研究的表面纹理方面将被整合，以获得生物活性材料，这些材料可用于在器官构建阶段以受控和时间和空间依赖的方式研究药物、生长因子或细胞，同时在最终产品--一个自主器官--中完全去除。

In WP6 strategies will be developed to enable the induced and controlled degradation of polySia scaffolds. Polysialic acids can be specifically degraded by phage borne enzymes, the endosialidases. The goal of this study is to use molecular biological techniques to modulate the cleavage characteristics of endosialidases and to develop strategies that allow site directed targeting and inactivation of the enzymes. Biomimetic mineralization techniques will be applied to provide materials with improved chemical stability and reduced sensitivity against the degrading enzymes. Certain mineral phases, for example iron oxides or hydroxides, can yield additional clues for a specific placement of the scaffolds. Organic- and polymer-chemical aspects studied in WP2 and WP3 as well as aspects of surface texturing studied in WP4 and WP5 will be integrated to obtain bioactive materials that can be used to deliberate drugs, growth factors, or cells in a controlled and time and space dependent manner during the organ construction phase, while becoming completely removed in the final product, an autonomous organ.