Studies on the controlled degradation of polySia scaffolds

在WP6中，将开发策略来诱导和控制polySia支架的降解。多唾液酸可以被噬菌体携带的酶，内唾液酸酶特异性降解。本研究的目的是利用分子生物学技术来调节内醛苷酶的裂解特性，并制定策略，使酶的定点靶向和失活。仿生矿化技术将用于提高材料的化学稳定性和降低对降解酶的敏感性。某些矿物相，例如氧化铁或氢氧化物，可以为支架的特定位置提供额外的线索。在WP2和WP3中研究的有机化学和聚合物化学方面，以及WP4和WP5中研究的表面纹理方面，将被整合在一起，以获得生物活性材料，这些生物活性材料可以在器官构建阶段以可控的、时空依赖的方式来降解药物、生长因子或细胞，而在最终产品中被完全去除，成为一个自主的器官。

In WP6 strategies will be developed to enable the induced and controlled degradation of polySia scaffolds. Polysialic acids can be specifically degraded by phage borne enzymes, the endosialidases. The goal of this study is to use molecular biological techniques to modulate the cleavage characteristics of endosialidases and to develop strategies that allow site directed targeting and inactivation of the enzymes. Biomimetic mineralization techniques will be applied to provide materials with improved chemical stability and reduced sensitivity against the degrading enzymes. Certain mineral phases, for example iron oxides or hydroxides, can yield additional clues for a specific placement of the scaffolds. Organic- and polymer-chemical aspects studied in WP2 and WP3 as well as aspects of surface texturing studied in WP4 and WP5 will be integrated to obtain bioactive materials that can be used to deliberate drugs, growth factors, or cells in a controlled and time and space dependent manner during the organ construction phase, while becoming completely removed in the final product, an autonomous organ.