Biogeographische, genetische und funktionelle Diversität von pflanzenwachstumsfördernden, oberirdisch auf Pflanzen vorkommenden Methylotrophen

植物地上促植物甲基营养菌的生物地理、遗传和功能多样性

• 批准号: 60761548
• 负责人: Professor Dr.-Ing. Peter Kämpfer
• 金额: --
• 依托单位: Institut für Angewandte Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Infrastructure Priority Programmes
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/60761548?language=en
• 关键词: Biogeographische; genetische; und; funktionelle; Diversit

Bacteria are by far the most dominant group on plants, and a high diversity of genera and species can be detected even on single plants. Only few studies have dealt with microbial succession patterns so far. Especially colonization patterns at the beginning of a growing season are still not very well known, as well as the spatial pattern of colonization, although it is known that bacteria can colonize leaves by air, soil, seeds or debris from previous crops. The objective of this project is to analyse the colonization pattern of plants by bacteria in dependence of plant species, land use intensity and geographic location. The succession of bacterial genera and species on plants will be studied as well as the spatial pattern of colonization, divided in upper, middle and lower plant leaf area. A combined cultivation dependent and –independent approach will be used to determine the bacterial species present on the leaves at different points in time at the beginning of the colonization process. Plants will be grown sterile and will be released into the field before sampling. All in all, about 450 plant leaves will be sampled from two plant species on areas with different land use intensity in all three exploratories. Samples will be analysed by total cell count, live/dead count, isolation, cloning and sequence analysis of 16S rRNA genes. Auxin will be measured for isolates. Additionally, the spatial patterns of bacterial communities on single leaves will be studied with fluorescent in situ hybridization.

细菌是植物上最具优势的类群，即使在单个植物上也可以检测到高度多样性的属和种。到目前为止，只有少数研究涉及微生物的演替模式。特别是在生长季节开始时的定殖模式以及定殖的空间模式仍然不是很清楚，尽管已知细菌可以通过空气、土壤、种子或来自先前作物的碎片定殖在叶子上。本项目的目标是分析细菌在植物中的定殖模式，这取决于植物种类、土地利用强度和地理位置。将研究植物上细菌属和种的演替以及定殖的空间格局，分为上、中、下植物叶面积。将使用组合的培养依赖性和非依赖性方法来确定在定殖过程开始时的不同时间点存在于叶片上的细菌种类。植物将无菌生长，并在取样前被释放到田间。总的来说，在所有三个探测站中，将从两种植物中采集大约450片植物叶片，这些植物叶片位于不同土地利用强度的区域。将通过总细胞计数、活/死细胞计数、16 S rRNA基因的分离、克隆和序列分析对样本进行分析。将测量分离株的生长素。此外，还将利用荧光原位杂交技术研究单叶片上细菌群落的空间格局。