Microdomain calcium signaling in secretory protozoan cell: Identification and functional localization of membrane proteins in calcium stores of Parameciums cells

分泌性原生动物细胞中的微域钙信号传导：草履虫细胞钙库中膜蛋白的鉴定和功能定位

• 批准号: 62154266
• 负责人: Professor Dr. Helmut Plattner
• 金额: --
• 依托单位: Fachbereich Biologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/62154266?language=en
• 关键词: Microdomain; calcium; signaling; secretory; protozoan

Signaling by Ca2+ involves Ca2+-influx as well as Ca2+ release channels in store membranes. Upon stimulation, Ca2+ signals have to be precisely localized, i.e., colocalized with target molecules to generate a quick response. So far, we have found in the Paramecium tetraurelia genome data base six different types of Ca2+-release channels (ryanodine and !P3 receptors, and mixed types without precedent-to be preferentially analyzed). Some are unexpectedly localized to the osmoregulatory system, others to the Endoplasmic Reticulum or to cortical Ca-stores. Understanding this complex situation requires detailed characterization and localization of Ca2+- channels and of target structures.

Ca ~（2+）的信号传导涉及贮藏膜中的Ca ~（2+）内流以及Ca ~（2+）释放通道。在刺激时，Ca 2+信号必须精确定位，即，与靶分子共定位以产生快速反应。到目前为止，我们已经在草履虫基因组数据库中发现了六种不同类型的钙释放通道（ryanodine和！P3受体，以及没有先例的混合型-优先分析）。有些出乎意料地定位于内质网调节系统，其他内质网或皮质钙库。理解这种复杂的情况需要详细的表征和定位的Ca 2+通道和目标结构。