Functions of Reggie proteins and microdomains

细胞膜是一种高度组织化的结构，在其他专门化区域中，它拥有微域，通过形成蛋白质簇来增强细胞信号的有效性。Reggie-1和-2为此类微域中的一种形成低聚支架，即Reggie微域。在淋巴细胞和神经元中，Reggie微域对于信号复合体的局部组装、调节肌动蛋白的细胞骨架动力学和协调细胞内的PrP功能至关重要。此外，Reggie微区的囊泡运输显然为内吞和胞吐定义了一条新的囊泡途径。在这里，我们将分析1.囊泡运输、相关的货物分子和轴突运输，以了解微域的形成和维持的动力学；2.生长锥的运动和延伸，以揭示RegGes在神经元分化和轴突再生中的功能；3.RegGes的作用及其与PrP的相互作用，在细胞接触和局灶性黏附形成中的作用；4.RegGys在鱼类和哺乳动物体内轴突生长和再生中的作用。最后，我们将把哺乳动物Reggie的功能与细菌、植物和果蝇中的Reggie样蛋白联系起来，并试图与Reggie簇的成员合作解决Reggie的膜相互作用和结构问题。

The cellular plasma membrane is a highly organized structure and posseses, among other specialized regions, microdomains which enhance cell-signaling efficacy through protein cluster formation. Reggie-1 and -2 form oligomeric scaffolds for one type of such microdomains, the Reggie microdomains. Reggie microdomains are crucial for the localized assembly of signalling complexes regulating actin cytoskeleton dynamics and coordinating cellular prion protein (PrP) function in lymphocytes and neurons. Furthermore, vesicular trafficking of reggie microdomains apparently defines a novel vesicular pathway for endo- and exocytosis. Here, we will analyse 1. vesicular trafficking, associated cargo molecules and axonal transport to understand the dynamics of microdomain formation and maintenance, 2. growth cone motility and elongation to uncover the function of the Reggies during neuronal differentiation and axon regeneration, 3. the role of Reggies and their interaction with PrP in cell contact and focal adhesion formation, 4. the role of Reggies during axon growth and regeneration in fish and mammals in vivo. Finally, we will correlate the function of mammalian Reggies with Reggie-like proteins in bacteria, plants and Drosophila and attempt to solve membrane interaction and structure of Reggies in collaboration with the members of the Reggie cluster.