Functions of Reggie proteins and microdomains

细胞膜是一个高度组织化的结构，在其他特殊区域中，具有微结构域，通过蛋白质簇形成增强细胞信号传导的功效。Reggie-1和-2形成一种微结构域的低聚支架，即Reggie微结构域。Reggie微结构域在淋巴细胞和神经元中对调节肌动蛋白细胞骨架动力学和协调细胞朊蛋白（PrP）功能的信号复合物的局部组装至关重要。此外，雷吉微结构域的囊泡运输显然为胞内和胞外作用定义了一种新的囊泡途径。在这里，我们将分析1。囊泡运输，相关的货物分子和轴突运输，以了解微结构域形成和维持的动力学。生长锥运动和伸长，揭示Reggies在神经元分化和轴突再生中的功能；Reggies在细胞接触和局灶黏附形成中的作用及其与PrP的相互作用；Reggies在鱼类和哺乳动物体内轴突生长和再生中的作用。最后，我们将把哺乳动物Reggies的功能与细菌、植物和果蝇中的Reggies样蛋白联系起来，并试图与Reggies簇成员合作解决Reggies的膜相互作用和结构问题。

The cellular plasma membrane is a highly organized structure and posseses, among other specialized regions, microdomains which enhance cell-signaling efficacy through protein cluster formation. Reggie-1 and -2 form oligomeric scaffolds for one type of such microdomains, the Reggie microdomains. Reggie microdomains are crucial for the localized assembly of signalling complexes regulating actin cytoskeleton dynamics and coordinating cellular prion protein (PrP) function in lymphocytes and neurons. Furthermore, vesicular trafficking of reggie microdomains apparently defines a novel vesicular pathway for endo- and exocytosis. Here, we will analyse 1. vesicular trafficking, associated cargo molecules and axonal transport to understand the dynamics of microdomain formation and maintenance, 2. growth cone motility and elongation to uncover the function of the Reggies during neuronal differentiation and axon regeneration, 3. the role of Reggies and their interaction with PrP in cell contact and focal adhesion formation, 4. the role of Reggies during axon growth and regeneration in fish and mammals in vivo. Finally, we will correlate the function of mammalian Reggies with Reggie-like proteins in bacteria, plants and Drosophila and attempt to solve membrane interaction and structure of Reggies in collaboration with the members of the Reggie cluster.