Functions of Reggie proteins and microdomains

Reggie 蛋白和微结构域的功能

• 批准号: 74892969
• 负责人: Professor Dr. Helmut Plattner
• 金额: --
• 依托单位: Fachbereich Biologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/74892969?language=en
• 关键词: Functions; Reggie; proteins; microdomains

The cellular plasma membrane is a highly organized structure and posseses, among other specialized regions, microdomains which enhance cell-signaling efficacy through protein cluster formation. Reggie-1 and -2 form oligomeric scaffolds for one type of such microdomains, the Reggie microdomains. Reggie microdomains are crucial for the localized assembly of signalling complexes regulating actin cytoskeleton dynamics and coordinating cellular prion protein (PrP) function in lymphocytes and neurons. Furthermore, vesicular trafficking of reggie microdomains apparently defines a novel vesicular pathway for endo- and exocytosis. Here, we will analyse 1. vesicular trafficking, associated cargo molecules and axonal transport to understand the dynamics of microdomain formation and maintenance, 2. growth cone motility and elongation to uncover the function of the Reggies during neuronal differentiation and axon regeneration, 3. the role of Reggies and their interaction with PrP in cell contact and focal adhesion formation, 4. the role of Reggies during axon growth and regeneration in fish and mammals in vivo. Finally, we will correlate the function of mammalian Reggies with Reggie-like proteins in bacteria, plants and Drosophila and attempt to solve membrane interaction and structure of Reggies in collaboration with the members of the Reggie cluster.

细胞质膜是一种高度组织化的结构，除其他特殊区域外，还拥有微结构域，这些微结构域可通过蛋白质簇的形成增强细胞信号传导的功效。 Reggie-1 和-2 形成一种此类微结构域（Reggie 微结构域）的寡聚支架。 Reggie 微结构域对于调节肌动蛋白细胞骨架动力学和协调淋巴细胞和神经元中的细胞朊病毒蛋白 (PrP) 功能的信号复合物的局部组装至关重要。 此外，雷吉微结构域的囊泡运输显然定义了一种新的胞吞和胞吐作用的囊泡途径。在这里，我们将分析 1. 囊泡运输、相关货物分子和轴突运输，以了解微域形成和维持的动态，2. 生长锥运动和伸长，以揭示 Reggies 在神经元分化和轴突再生过程中的功能，3. Reggies 的作用及其与 PrP 的相互作用在细胞接触和粘着斑形成中的作用，4. Reggies 在轴突生长过程中的作用 以及鱼类和哺乳动物体内的再生。最后，我们将哺乳动物 Reggie 的功能与细菌、植物和果蝇中的 Reggie 样​​蛋白联系起来，并尝试与 Reggie 簇成员合作解决 Reggie 的膜相互作用和结构。