Identification of nuclear encoded genes involved in 5' end processing of mitochondrial mRNA in Arabidopsis thaliana

拟南芥线粒体 mRNA 5 端加工相关核编码基因的鉴定

• 批准号: 64652646
• 负责人: Professor Dr. Stefan Binder
• 金额: --
• 依托单位: Institut für Molekularbiologie und Biotechnologie der Prokaryoten
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/64652646?language=en
• 关键词: Identification; nuclear; encoded; genes; involved

In the proposed project genes involved in 5 end processing of plant mitochondrial transcripts will be identified by a forward genetic approach. The groundwork for this project is the analysis of major mitochondrial mRNA ends in three different ecotypes of Arabidopsis thaliana. This investigation revealed ecotype-specific 5’ mRNA polymorphisms for the mitochondrial genes nad9, nad4, ccmB and rpl5. Analysis of corresponding F1 hybrids assigned these polymorphisms to different nuclear encoded genes. Now the naturally occurring genetic variation in the model species A. thaliana will be used for map-based cloning of these nuclear genes encoding mitochondrial RNA processing factors (RPF). The highly valuable system of natural variation is presently the sole alternative to the very limited cytoplasmic male sterility/restorer of fertility system, which is not found for A. thaliana. To identify further ecotype-specific variations a comprehensive descriptive analysis of 5’ processing of mRNA derived from gene encoding subunits of complex I (nad genes) will be initiated in parallel. This investigation will yield more definite information about the processing events and of RNA intermediates.

在拟议的项目中，参与植物线粒体转录本5个末端加工的基因将通过正向遗传方法进行识别。本项目的基础工作是分析三种不同生态型拟南芥的主要线粒体mRNA末端。本研究揭示了线粒体基因nad9、nad4、ccmb和rpl5的生态型特异性5‘m RNA多态。对相应的F1杂交种的分析将这些多态分配给不同的核编码基因。现在，模式物种A.thaliana中自然发生的遗传变异将被用于基于地图的这些编码线粒体RNA加工因子(RPF)的核基因的克隆。具有很高价值的自然变异系统是目前唯一可以替代非常有限的细胞质雄性不育/育性恢复系统的系统，而这一系统在拟南芥中还没有发现。为了确定进一步的生态型变异，将同时启动对来自复合体I(NAD基因)编码亚单位基因的5‘端处理的mRNA的全面描述性分析。这项调查将产生关于加工事件和RNA中间体的更确定的信息。

项目成果

In Arabidopsis thaliana two co-adapted cyto-nuclear systems correlate with distinct ccmC transcript sizes.

在拟南芥中，两个共同适应的细胞核系统与不同的 ccmC 转录本大小相关

• DOI: 10.1111/tpj.12724
• 发表时间: 2015
• 期刊: The Plant journal : for cell and molecular biology
• 作者: Jonietz;Binder
• 通讯作者: Binder

Mitochondrial transcript length polymorphisms are a widespread phenomenon in Arabidopsis thaliana

线粒体转录本长度多态性是拟南芥中普遍存在的现象

• DOI: 10.1007/s11103-012-9993-z
• 发表时间: 2013
• 期刊: Plant Molecular Biology
• 影响因子: 5.1
• 作者: Steinhilber;Jonietz;Binder
• 通讯作者: Binder

In Arabidopsis thaliana distinct alleles encoding mitochondrial RNA PROCESSING FACTOR 4 support the generation of additional 5′ termini of ccmB transcripts

• DOI: 10.1007/s11103-017-0591-y
• 发表时间: 2017-02
• 期刊: Plant Molecular Biology
• 影响因子: 5.1
• 作者: Katrin Stoll;Christian Jonietz;S. Schleicher;C. C. des Francs-Small-C.;I. Small;S. Binder

RNA Processing Factor 7 and Polynucleotide Phosphorylase Are Necessary for Processing and Stability of nad2 mRNA in Arabidopsis Mitochondria

• DOI: 10.4161/rna.29781
• 发表时间: 2014-07-01
• 期刊: RNA BIOLOGY
• 影响因子: 4.1
• 作者: Stoll, Birgit;Zendler, Daniel;Binder, Stefan
• 通讯作者: Binder, Stefan