A STUDY OF THE DISTRIBUTION OF AXONS CONTAINING SUBSTANCE P AND THE LOCALIZATION OF NEUROKININ-1 RECEPTOR IN TEMPOROMANDIBULAR JOINT ARTHRITIS OF THE RAT.

1.免疫光镜和免疫电镜观察正常大鼠颞下颌关节NOS的定位：6周龄雄性Wistar大鼠麻醉后，经心脏灌注固定液，解剖出颞下颌关节。脱钙后，获得冷冻切片并进行免疫光镜和免疫电镜观察。采用eNOS或iNOS抗体ABC法。eNOS和iNOS的免疫反应性被发现在滑膜衬里细胞的前部和后部的面向上室的颞下颌关节腔，并在前面和侧面的部分滑膜面向下室。eNOS在滑膜血管内皮细胞中也有表达。免疫电镜观察显示，eNOS和iNOS的免疫反应主要见于滑膜衬里细胞的A型细胞。在这些A型细胞中，eNOS和iNOS的免疫反应产物主要分布于胞浆、囊泡和空泡结构中.佐剂性关节炎大鼠颞下颌关节NOS定位的免疫光镜观察：7周龄雄性刘易斯大鼠注射佐剂诱导关节炎。以与上述方法相同的方式进行eNOS和iNOS的免疫光显微镜观察。eNOS和iNOS在炎症TMJ滑膜衬里细胞中的免疫反应性定位与正常关节相似。eNOS或iNOS的免疫反应性被发现在增殖的滑膜衬里细胞面临的上或下关节腔。eNOS的免疫反应性的强度，但是，强于iNOS。虽然没有明显的差异，发现在正常大鼠和关节炎大鼠之间的滑膜衬里细胞的eNOS的免疫反应性的强度，iNOS的免疫反应性在关节炎大鼠减少。

1. Immunolight- and immunoelectron-microscopic observation of the localization of NOSs in the normal rat TMJ : Six-week-old male Wistar rats were anesthetized and perfused transcardically with fixative, and TMJs were dissected out. After decalcification, frozen sections were obtained and submitted to immunolight- and immunoelectron-microscopic observation. ABC method with antibodies for eNOS or iNOS was utilized. Immunoreactivity for eNOS and iNOS was found in the synovial lining cells in the anterior and posterior portions of the synovium facing the upper compartment of the TMJ cavity, and in the anterior and lateral portions of the synovium facing of the lower compartment. eNOS was also detected in the endothelial cells of blood vessels close to the synovium. Immunoelectron-microscopic observation revealed that immunoreactivity for eNOS and iNOS was mainly found in the type A cells of the synovial lining cells. In these type A cells, immunoreactive products for eNOS and iNOS were distributed mainly in the cytoplasm, and in the vesicular and vacuolar structures.2. Immunolight-microscopic observation of the localization of NOSs in the rat TMJ with adjuvant-induced arthritis : Arthritis was induced by the injection of the adjuvant to 7-week-old male Lewis rats. immunolight-microscopic observation for eNOS and iNOS was performed in the same manner as the above mentioned method. The localization of immunoreactivity for eNOS and iNOS in the synovial lining cells of the inflamed TMJ was similar to that of the normal joint. Immunoreactivity for eNOS or iNOS was found in proliferated synovial lining cells facing the upper or lower joint compartment. The strength of immunoreactivity for eNOS was, however, stronger than that for iNOS.Although no clear difference was found in the strength of immunoreactivity for eNOS in the synovial lining cells between the normal rat and the arthritis rat, immunoreactivity for iNOS was reduced in the arthritis rat.