Unconventional ubiquitin chains as regulators of plant development

非常规泛素链作为植物发育的调节因子

• 批准号: 71963938
• 负责人: Professor Dr. Andreas Bachmair
• 金额: --
• 依托单位: Max F. Perutz Laboratories
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2008
• 资助国家: 德国
• 起止时间: 2007-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/71963938?language=en
• 关键词: Unconventional; ubiquitin; chains; regulators; plant

In addition to the well-established role in proteolysis, the small modifier protein ubiquitin has important functions as a reversible tag in animals and in yeast. Such functions are, however, largely unexplored in plants. Previous work by the applicant indicated that Arabidopsis plants with lesions in two related ubiquitin ligases show complete loss of apical dominance, i. e. mutant plants do not have a main shoot that suppresses the outgrowth of additional shoots on the same plant. The ligases were characterized to have specificity for formation of ubiquitin chains linked via Lys 63 of ubiquitin. Such chains do not target substrates for proteolysis, but facilitate novel protein interactions as molecular switches. We want to investigate the integration of the ligases into developmental processes by identification of substrates, and by in-depth investigation of those aspects of plant hormone metabolism that were shown to be regulated by these ubiquitin ligases.

除了在蛋白水解中明确的作用外，小修饰蛋白泛素还具有作为动物和酵母中的可逆标签的重要功能。然而，植物中的这些功能在很大程度上尚未被探索。申请人之前的工作表明，在两种相关的泛素连接酶中具有损伤的拟南芥植物显示出顶端优势的完全丧失，即。 e.突变植物没有主枝来抑制同一植物上其他枝条的生长。连接酶的特点是对通过泛素第 63 位赖氨酸连接的泛素链的形成具有特异性。此类链不针对蛋白水解底物，但作为分子开关促进新的蛋白质相互作用。我们希望通过鉴定底物并深入研究被证明受这些泛素连接酶调节的植物激素代谢的那些方面来研究连接酶在发育过程中的整合。