The role of the N-end rule pathway in controlling plant response to the environment

在不可预测的生长条件下，对植物进行操纵以保证产量的稳定性，对于应对气候变化的影响至关重要，因为气候变化增加了农业环境的不确定性。通过蛋白水解选择性和有条件地去除调节蛋白正在成为植物的主要调节原理。n -环境项目的目的是提供一个完整的机制理解的作用的n端规则途径的靶向蛋白水解在控制植物对环境的反应。为了实现这一目标，我们选择了代表国际领先的欧洲团队的合作伙伴，他们在该途径的多个方面具有互补的专业知识，包括该途径的生化基础、缺氧、应激的转录调节和代谢信号。该项目将汇集四个机构的六个研究小组，他们在基础分子植物科学、生物化学和化学方面具有互补的专业知识。n - environment联盟的研究方案将通过由四个联盟伙伴（三个由ERA-CAPS资助，一个联合实验室）执行的六个相互关联的工作包来实现。这些工作包的目标与三个基本问题有关，这些问题是在整合了最近由联盟成员发现的靶向蛋白水解的n端规则途径作为植物发育和对环境响应的主要调节剂的作用后产生的。问题1:n端规则途径的底物和酶成分是什么？问题2:n端规则通路是如何整合到细胞信号通路中的？问题3:n端规则通路在植物对生物和非生物环境的响应中发挥了多大的作用？拟议的研究具有高度创新的可测量的结果，解决了植物生物学这一新发现的领域，并将揭示：调节蛋白质稳定性的新机制，环境胁迫传感的新机制，蛋白质在胁迫传感中的新功能，欧盟主要作物番茄n端规则的重要性。这一快速发展的植物分子科学领域由n - environment成员（包括底物的发现、进入n端规则途径的方法、途径的生化成分）领导，加上联盟内大量独特资源的可用性，使得n -环境的提议具有高度的新颖性和及时性。这一合作计划的协同价值将是开发和利用一个对农业至关重要的高度新颖的植物生物学领域，在这个领域，欧洲有能力通过ERA-CAPS的资助走在世界前列。

Manipulation of plants to provide stability of yield under unpredictable growth conditions will be essential to respond to the effect of climate change in increasing the uncertainty of the agricultural environment. Selective and conditional removal of regulator proteins by proteolysis is emerging as a major regulatory principle in plants. The aim of the N-vironment project is to provide a complete mechanistic understanding of the role of the N-end rule pathway of targeted proteolysis in controlling plant responses to the environment. To achieve this goal consortium partners have been selected that represent internationally leading European teams with complementary expertise in the pathway´s multiple facets, including the biochemical basis of the pathway, hypoxia, transcriptional regulation of stress and metabolic signalling. The project will bring together six research groups with complementary expertise in fundamental molecular plant science, biochemistry and chemistry, in four institutions. The research programme of the N-vironment consortium will be achieved through six inter-related Work Packages (WPs) carried out by the four consortium partners (three funded by ERA-CAPS, one associated laboratory). The objectives of these work packages are related to three fundamental questions that arose after integration of the recent discoveries by consortium members of the role of the N-end rule pathway of targeted proteolysis as a major regulator of plant development and response to the environment. Question 1: What are the substrates and enzymatic components of the N-end rule pathway? Question 2: How is the N-end rule pathway integrated into cellular signaling pathways? Question 3: What is the extent of the role of the N-end rule pathway in plant response to the biotic and abiotic environment? The proposed research has highly innovative measurable outcomes that address this newly discovered area of plant biology, and will uncover: New mechanisms regulating protein stability, new mechanisms of environmental stress sensing, new functions for proteins in stress sensing, the importance of the N-end rule in a key EU crop, tomato. This fast-developing area of plant molecular science is led by N-vironment members (including discovery of substrates, methods of entry into the N-end rule pathway, biochemical components of the pathway), and together with the availability of a large number of unique resources within the consortium, makes the N-vironment proposal highly novel and timely. The synergistic value of this collaborative programme will be the development and exploitation of a highly novel area of plant biology of key importance to agriculture, in which Europe has the capacity to take a world lead through ERA-CAPS funding.