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Regulation of carbon metabolism in staphylococci: The impact of catabolite control protein A and related factors on pathogenicity of Staphylococcus aureus and Staphylococcus epidermidis

葡萄球菌碳代谢的调控：分解代谢控制蛋白A及相关因素对金黄色葡萄球菌和表皮葡萄球菌致病性的影响

基本信息

批准号：
71758911
负责人：
Professor Dr. Markus Bischoff
金额：
--
依托单位：
Institut für Medizinische Mikrobiologie und Hygiene
依托单位国家：
德国
项目类别：
Priority Programmes
财政年份：
2008
资助国家：
德国
起止时间：
2007-12-31 至 2015-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/71758911?language=en
关键词：
Regulation carbon metabolism staphylococci impact

项目摘要

Carbon catabolism-affecting factors have been shown in many bacteria to influence virulence and pathogenesis. We recently identified the catabolite control protein A (CcpA) in Staphylococcus aureus, which is thought to be the major regulator of carbon catabolite repression (CCR) in Gram-positive bacteria. During the first funding period, we could show that the deletion of this regulator affected carbon metabolism, virulence determinant production, and infectivity of this pathogen. Aim of the second funding period of this project is now the characterization of the regulatory control of this DNA-binding protein in S. aureus, its impact on infections that are frequent in diabetic patients, and its role in persistence within its host. We will also try to identify the transcriptional changes that occur during the early adaptation processes of S. aureus within its host, and we are planning to characterize further carbon metabolism-affecting regulators of this organism, such as CitR, Hpr, and CodY. The proposed work will yield detailed insights into metabolic processes that are effective within S. aureus and its host during invasion and persistence, and into regulatory circuits that link carbon metabolism and virulence in this pathogen.

许多细菌中碳分解代谢影响因素已被证明可以影响毒力和发病机制。我们最近在金黄色葡萄球菌中发现了分解代谢物控制蛋白 A (CcpA)，它被认为是革兰氏阳性菌中碳分解代谢物抑制 (CCR) 的主要调节因子。在第一个资助期间，我们可以证明该调节因子的缺失会影响该病原体的碳代谢、毒力决定簇的产生和感染性。该项目第二期资助的目标是表征金黄色葡萄球菌中这种 DNA 结合蛋白的调节控制、其对糖尿病患者常见感染的影响以及其在宿主体内持续存在的作用。我们还将尝试确定金黄色葡萄球菌在其宿主内的早期适应过程中发生的转录变化，并且我们计划进一步表征该生物体影响碳代谢的调节因子，例如 CitR、Hpr 和 CodY。拟议的工作将详细了解金黄色葡萄球菌及其宿主在入侵和持久性过程中有效的代谢过程，以及将碳代谢和该病原体的毒力联系起来的调节回路。