Comparative analysis of distinct seed dormancy mechanisms within the genus Lepidium (cress) and the evolution of the Brassicaceae DOG1 dormancy genes

拟南芥DO1(Delay Of GERMINATION1)是利用自然休眠遗传变异克隆的第一个种子休眠特异基因。Dog1基因是拟南芥休眠所必需的，但它的序列并不提供功能信息。我们希望通过野生型和突变型种子的比较定量分析来研究DOG1相关的全种子蛋白质组。除了Brassica Dog1同源基因的序列外，对其他物种中的Dog1一无所知。我们已经从大麦中克隆了一个DOG1同源基因，它是大麦的近亲，种子较大，将被用来分析种子组织特异性蛋白质组。独脚菜属(水芹属)提供了一个单系分支，有不同休眠的物种(非休眠、被毛或胚胎休眠的种子)。我们将研究Dog1在不同休眠状态下的表达。我们将通过RNAi和正义转化来改变它们的Dog1的表达，并研究它们对转基因独行草种子休眠的影响。

DOG1 (DELAY OF GERMINATION1) of Arabidopsis thaliana is the first seed dormancy-specific gene cloned by exploiting natural genetic variation in dormancy. The DOG1 gene is essential for Arabidopsis dormancy, but its sequence does not provide functional information. We want to investigate the DOG1-related whole-seed proteome by comparative quantitative analysis of wildtype and mutant seeds. Except for the sequence of a Brassica DOG1 homolog, nothing is known about DOG1 in other species. We have cloned a DOG1 homolog from Lepidium sativum, a close relative of A. thaliana with bigger seeds which will be used to analyse the seed-tissue-specific proteome. The genus Lepidium (cress) provides a monophyletic clade with species that differ in dormancy (non-, coat-, or embryo-dormant seeds). We will study DOG1 expression of Lepidium seeds with different dormancy. We will alter their DOG1 expression by RNAi and sense transformation and study the effect on dormancy of the transgenic Lepidium seeds.