The origin of nitergic nerve terminals in the guinea-pig heart

豚鼠心脏含氮神经末梢的起源

• 批准号: 08680809
• 负责人: TANAKA Koichi
• 金额: $ 1.6万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08680809/
• 关键词: Cardiac Ganglia; Nitric oxide synthase; Vagus nerve; Guinea pig; Vagotomy

The origin of nerve fibers projecting to the guinea pig heart that contain nitric oxide synthase (NOS) was studied by unilateral cervical vagotomy. Three kinds of NOS-immunoreactive (NOS-ir) nerve fibers are distributed in the control guinea pig heart : the sparse network covering the right atrium, the basket-like endings around intracardiac neuronal cell bodies in the small ganglia located in the left atrium and the interatrial septum, and the axons situated in the septal region. The sparse network in the right atrium did not change after vagotomy of right or left side. In the wholemount preparations of right atrium, we often traced labeled axons from the somata to join the network covering the right atrium. Therefore, most of these networks of labeled fibers must be of intrinsic origin. Because the basket-like endings around neuronal cell bodies in the ganglia in the left atrium and the septum disappeared completely after vagotomy of left side, they seem to be parasympathetic preganglionic fibers come from the left vagus nerve. NOS-ir cell bodies and the positive fibers in the atrioventricular nodal region survived after vagotomy. All of such nerve fibers were unmyelinated axons. Therefore, they seem to be the postganglionic fibers arising from the ganglia located in the left atrium or the septum.

通过单侧颈部迷走神经切断术研究了投射到豚鼠心脏的含有一氧化氮合酶（NOS）的神经纤维的起源。在正常豚鼠心脏中分布有三种NOS免疫反应（NOS-ir）神经纤维：覆盖右心房的稀疏网络，位于左心房和房间隔的小神经节中围绕心内神经元胞体的篮状末梢，以及位于间隔区的轴突。左、右侧迷走神经切断后，右心房内的稀疏网状结构无明显变化。在右心房的整体标本中，我们经常追踪标记的轴突从胞体进入覆盖右心房的网络。因此，大多数这些标记的纤维网络必须是内在的起源。由于左侧迷走神经切断后，左心房神经节和隔神经元胞体周围的篮状末梢完全消失，它们似乎是来自左侧迷走神经的副交感节前纤维。迷走神经切断后，房室结区NOS阳性胞体和阳性纤维仍存活。所有这些神经纤维都是无髓轴突。因此，它们似乎是从位于左心房或隔的神经节发出的节后纤维。

项目成果

Hanazawa, T., Tanaka, K., et al.: "Distribution and origin of nitric oxide synthase-containing nerve fibers in human nasal mucosa." Acta Otolaryngol (Stockh). 117. 735-737 (1997)

Hanazawa, T.、Tanaka, K. 等人：“人鼻粘膜中含有一氧化氮合酶的神经纤维的分布和起源。”

Tanaka, and Chiba, T.: "Microvascular organization of sympathetic ganglia,with special reference to SIF cells" Microscopy Research and Technigue. 35. 137-145 (1996)

Tanaka 和 Chiba, T.：“交感神经节的微血管组织，特别是 SIF 细胞”显微镜研究与技术。

Suseki, K., Tanaka, K.他: "CGRP-immunoreactive nerve fibers projecting lumbar facet joints through paravertebral sympathetic trunk in the rat" Neurosci.Letl.221. 41-44 (1996)

Suseki, K.、Tanaka, K. 等人：“CGRP 免疫反应性神经纤维通过大鼠椎旁交感干投射腰椎小关节” Neurosci.Letl.221 (1996)。

Suseki,K., Tanaka,K.他: "CGRP- immunoitactive nerve fibers projecting lumbar facet joints through paraovertebral sympathetic trunk in rats" Neurosci.Lett.221. 41-44 (1996)

Suseki, K.、Tanaka, K. 等人：“CGRP-免疫活性神经纤维通过大鼠椎旁交感干投射腰椎小关节” Neurosci.Lett.221 (1996)。

Hanazawa, T., Tanaka, K.他: "Distribution and origin of nitric oxide syuthase-coutaining nevve fibers in human nasal mucosa" Acta Otolaryngol(Stockh). 117. 735-737 (1997)

Hanazawa, T., Tanaka, K. 等人：“人鼻粘膜中一氧化氮合成酶抑制色素纤维的分布和起源”Acta Otolaryngol(Stockh) 117. 735-737 (1997)。