The sensory innervation of the parietal peritoneum in the rat

用氟金轴突转运法研究了支配腹膜顶骨的感觉神经元的分布。示踪剂被注射到腹膜顶骨和横膈膜的各个部位。存活10 d后，切除双侧C2 ~ S6背根神经节及结节性神经节。将这些神经节的低温恒温切片装在玻璃载玻片上，用荧光显微镜观察。当示踪剂放置在覆盖腹壁的腹膜上时，仅在同侧背根神经节中观察到标记神经元。在膈中央放置示踪剂后，标记两侧结节和颈背根神经节的少量神经元。当氟金应用于膈外周部分时，结节神经节呈阴性，T6 ~ T12背根神经节呈阳性。这些结果表明，腹膜顶膜大部分接受来自背根神经节的感觉神经。应用抗血清抗神经元标志物PGP9.5免疫组化方法研究这些支配腹膜顶骨的感觉神经纤维的形态。这些轴突经常形成波浪形束，并向各个方向移动。这些束包含许多有髓和无髓轴突。我们观察到单个神经纤维从这些神经束分支出来，向腹腔延伸。单个髓鞘纤维与几个雪旺细胞一起，从表面下的髓鞘中逃逸出来，分成几个突起到达腹腔。

The distribution of sensory neurons innervating the parietal peritoneum was studied using axonal transport of fluoro-gold. The tracer was injected to various parts of parietal peritoneum and the diaphragm. After ten days of survival period bilateral dorsal root ganglia from C2 to S6, and the nodose ganglia were dissected. The cryostat sections of these ganglia were mounted on glass slides and observed with a fluorescence microscope. In cases where the tracer was placed on the peritoneum covering the abdominal wall, labeled neurons were observed only in the ipsilateral dorsal root ganglia. A small number of neurons in nodose and cervical dorsal root ganglia of both sides were labeled after placing the tracer on the central part of diaphragm. When fluoro-gold was applied to the peripheral part of the diaphragm, nodose ganglion was negative, and dorsal root ganglia from T6 to T12 were positive. These results suggest that most of the parietal peritoneum receives sensory nerves from dorsal root ganglia.The morphology of these sensory nerve fibers supplying the parietal peritoneum was studied by immunohistochemistry using antisera against neuronal marker, PGP9.5. These axons often formed wavy bundles and ran in various directions. These bundles contained many myelinated and unmyelinated axons. We observed single nerve fibers branching from these bundles, running toward the peritoneal cavity. The single myelinate fibers ran with several Schwann cells, escaped from the myelin sheeth at just beneath the surface, devided into a few processes to reach the peritoneal cavity.