Excellent biocompatibility of MPC polymers as biomaterials evaluated by fibroblast and HL-60 cells.

细胞附着在生物材料表面是由吸附在那里的蛋白质引发的。当蛋白质吸附被阻止时，细胞附着必须最小化。甲基丙烯酰氧乙基磷酰胆碱（MPC）的共聚物由于表面上的磷酰胆碱极性基团而对磷脂具有亲和力。我们发现MPC表面抑制HL-60细胞的粘附，而且它们不促进细胞因子的产生，因此它们是用于血液接触装置的有用生物材料。该结果表明MPC表面不刺激细胞。另一方面，常规生物材料促进细胞因子在细胞中的积累。因此，MPC是一种对细胞无不良影响的优良生物材料。此外，该评价方法必须适用于检查生物材料的生物相容性。另一个有趣的结果是成纤维细胞不能攻击涂覆有MPC共聚物的表面。而当用Ne离子注入去除涂层时，它们可以在涂层上附着并增殖。也证实了此处的蛋白质吸附。

Attachment of cells to a biomaterial surface are initiated the protein adsorbed there. When protein adsorption are prevented, cellular attachment must be minimized. Copolymers of methacryloyloxyethyl phosphorylcholine (MPC) have affinity for phospholipids due to the phosphorylcholine polar groups on surface. It has been found that they are useful biomaterials for blood contacting devices as they do not induce platelet activation and blood coagulation.We realized that MPC surfaces inhibited attachment oh HL-60 cells and moreover they did not promote cytokine production. This result suggested that the MPC surface did not stimulate the cells. On the other hand conventional biomaterials promote accumulation of cytokine in the cells. We could conclude that MPC is very excellent biomaterials which do not give adverse effect on the cells. Further, this evaluation method must be applicable to examine biocompatibility of biomaterials. Another interesting result was fibroblast cell could not attack on the surface coated with MPC copolymers. On the other hand, when the coating was removed by Ne ion injection, they could attach and prolify there. Protein adsorption here was confirmed also.