Excellent biocompatibility of MPC polymers as biomaterials evaluated by fibroblast and HL-60 cells.

细胞附着在生物材料表面，蛋白质被吸附在那里。当阻止蛋白质吸附时，必须尽量减少细胞附着。甲基丙烯酰氧乙基磷酸胆碱（MPC）共聚物由于其表面的磷酸胆碱极性基团而对磷脂具有亲和力。由于它们不诱导血小板活化和血液凝固，因此已被发现是用于血液接触装置的有用生物材料。我们发现MPC表面抑制HL-60细胞的附着，而且它们不促进细胞因子的产生。这表明MPC表面对细胞没有刺激作用。另一方面，传统生物材料促进细胞因子的积累。因此，MPC是一种对细胞无不良影响的优良生物材料。此外，该评价方法必须适用于生物材料的生物相容性检验。另一个有趣的结果是成纤维细胞不能攻击包覆MPC共聚物的表面。另一方面，当注入氖离子去除涂层时，它们可以附着并增殖。蛋白质在这里的吸附也被证实。

Attachment of cells to a biomaterial surface are initiated the protein adsorbed there. When protein adsorption are prevented, cellular attachment must be minimized. Copolymers of methacryloyloxyethyl phosphorylcholine (MPC) have affinity for phospholipids due to the phosphorylcholine polar groups on surface. It has been found that they are useful biomaterials for blood contacting devices as they do not induce platelet activation and blood coagulation.We realized that MPC surfaces inhibited attachment oh HL-60 cells and moreover they did not promote cytokine production. This result suggested that the MPC surface did not stimulate the cells. On the other hand conventional biomaterials promote accumulation of cytokine in the cells. We could conclude that MPC is very excellent biomaterials which do not give adverse effect on the cells. Further, this evaluation method must be applicable to examine biocompatibility of biomaterials. Another interesting result was fibroblast cell could not attack on the surface coated with MPC copolymers. On the other hand, when the coating was removed by Ne ion injection, they could attach and prolify there. Protein adsorption here was confirmed also.