Excellent biocompatibility of MPC polymers as biomaterials evaluated by fibroblast and HL-60 cells.

当蛋白质吸附在生物材料表面时，细胞就开始附着在生物材料表面。当阻止蛋白质吸附时，必须将细胞附着降至最低。甲基丙烯酰氧乙基磷酰胆碱(MPC)共聚物由于表面含有磷酰胆碱极性基团，具有与磷脂的亲和力。我们发现，由于它们不会诱导血小板活化和血液凝固，它们是有用的血液接触设备的生物材料。我们意识到MPC表面抑制了HL-60细胞的附着，而且它们也没有促进细胞因子的产生。这一结果表明MPC表面对细胞没有刺激作用。另一方面，传统的生物材料促进细胞内细胞因子的积累。由此可见，MPC是一种对细胞无不良影响的优良生物材料。此外，该评价方法必须适用于检测生物材料的生物相容性。另一个有趣的结果是，成纤维细胞不能攻击涂有MPC共聚物的表面。另一方面，当用Ne离子注入去除涂层时，它们可以附着在那里并在那里生长。蛋白质在这里的吸附也得到了证实。

Attachment of cells to a biomaterial surface are initiated the protein adsorbed there. When protein adsorption are prevented, cellular attachment must be minimized. Copolymers of methacryloyloxyethyl phosphorylcholine (MPC) have affinity for phospholipids due to the phosphorylcholine polar groups on surface. It has been found that they are useful biomaterials for blood contacting devices as they do not induce platelet activation and blood coagulation.We realized that MPC surfaces inhibited attachment oh HL-60 cells and moreover they did not promote cytokine production. This result suggested that the MPC surface did not stimulate the cells. On the other hand conventional biomaterials promote accumulation of cytokine in the cells. We could conclude that MPC is very excellent biomaterials which do not give adverse effect on the cells. Further, this evaluation method must be applicable to examine biocompatibility of biomaterials. Another interesting result was fibroblast cell could not attack on the surface coated with MPC copolymers. On the other hand, when the coating was removed by Ne ion injection, they could attach and prolify there. Protein adsorption here was confirmed also.