Application of octacalcium phosphate for bone substitutes and a carrier for bone morphogenetic protein

磷酸八钙在骨替代物及骨形态发生蛋白载体中的应用

• 批准号: 09672035
• 负责人: KAMAKURA Shinji
• 金额: $ 2.3万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672035/
• 关键词: Bone; Bone Formation; Bone Repair; Octacalcium Phosphate; Calcium Phosphate

Synthetic octacalcium phosphate (OCP) enhances bone lbrmation if implanted into the subperiosteal region of murine bone. Such implanted QCP may be resorbed by the multinucleated giant cells which share some ultrastmctural characteristics with osteoclasts and replaced by bone with time. We hypothesized that OCP could be used as an effective bone substitute. To test this hypothesis, the present study was designed to investigate if bone repair in a rat skull defect is enhanced by the implantation of OCP.Rats were divided into two groups : OCP-treated animals and untreated controls. Six rats from each group were fixed at4, 12, and 24 weeks after implantation. A full-thickness standardized trephine defect was made in the parietal bone and synthetic OCP was implanted into the defect. After being examined radiographically, the specimens were decalcifled and processed for histology.OCP implantation significantly promoted bone repair compared to the controls. A statistical analysis showed an increase in the area of radiopacity within the skull defect between week 4 and week 12. Histologically, bone was formed on the implanted OCP and along the defeut margin atweek4. At week 12, the implanted OCP was surrounded by newly formed bone. At week 24, the defect was almost completely filled with bone. In the control, bone formation was observed only along the defect margin.The present results demonstrate that OCP could be used as an effective bone substitute.

人工合成的磷酸八钙（OCP）植入小鼠骨膜下可促进骨形成。这种植入的QCP可被多核巨细胞吸收，这些巨细胞与破骨细胞具有某些超微结构特征，并随着时间的推移被骨取代。我们假设OCP可以作为一种有效的骨替代物。为了验证这一假设，本研究的目的是调查，如果在大鼠颅骨缺损的骨修复是通过植入OCP增强。大鼠分为两组：OCP治疗的动物和未处理的对照组。在植入后4、12和24周每组6只大鼠固定。在顶骨中制作全层标准化环钻缺损，并将合成OCP植入缺损中。经X线检查后，标本脱钙并进行组织学处理。与对照组相比，OCP植入显著促进了骨修复。统计分析显示，在第4周和第12周之间，颅骨缺损内的不透射线面积增加。第4周组织学观察，骨形成于OCP上及沿着缺损边缘。在第12周，植入的OCP被新形成的骨包围。在第24周，缺损几乎完全被骨填充。对照组仅沿着缺损边缘有骨形成，表明OCP可作为一种有效的骨替代材料。

项目成果

Kagayama, M,Sasano, Y,Zhu, J-X,Hirata, M,Mizoguchi, I,Kamakura, S: "Epithelial rests colocalized with cementoblasts forming acellular cementum but not with cementoblasts forming cellular cementum." Acta Anat. 163. 1-9 (1998)

Kagayama，M，Sasano，Y，Zhu，J-X，Hirata，M，Mizoguchi，I，Kamakura，S：“上皮与形成无细胞牙骨质的成牙骨质细胞共定位，但不与形成细胞牙骨质的成牙骨质细胞共定位。”

Kagayama, M,Sasano, Y,Sato, H,Kamakura, S,Motegi, K,Mizoguchi, I: "Confocal microscopy of dentinal tubules in human tooth stained with alizarin red." Anat Embryol. (in press). (1998)

Kagayama，M，Sasano，Y，Sato，H，Kamakura，S，Motegi，K，Mizoguchi，I：“用茜素红染色的人牙齿牙本质小管的共聚焦显微镜。”

Sato S.et al.: "Morphologic changes in the elastic fibers of the temporomandibular joint after experimental disc perforation in the rabbit"Journal of Oral and Maxillofacial Surgery. 56. 753-758 (1998)

Sato S.等人：“兔子实验性椎间盘穿孔后颞下颌关节弹性纤维的形态变化”口腔颌面外科杂志。

Sasano Y.et al.: "Type X collagen is not localized in hypertrophic or calcified cartilage in the developing rat trachea"Anatomy and Embryology. 197. 399-403 (1998)

Sasano Y.等人：“X型胶原蛋白并不局限于发育中的大鼠气管的肥厚或钙化软骨中”解剖学和胚胎学。

Kagayama M,et al: "Confocal microscopy of dentinal tubules in human tooth stained with alizarin red." Anatomy and Embryology. (in press). (1998)

Kagayama M，等人：“用茜素红染色的人牙牙本质小管的共聚焦显微镜。”