Anti-tumor effect and mechanism of the peptide from Lactobacillus plantarum

小鼠移植P815肥大细胞瘤后，接种肿瘤抗原肽(P1a)和植物乳杆菌多肽，观察小鼠存活率。接种肿瘤抗原肽(P1a)的小鼠存活率为50%，接种植物乳杆菌多肽的小鼠存活率为50%；接种P815肥大细胞瘤小鼠后，每周两次接种肿瘤抗原肽(P1a)和胞壁二肽(MDP)，每周两次。观察小鼠脾细胞毒性T淋巴细胞(CTL)活性、白介素2(IL-2)产生情况、细胞毒T淋巴细胞(CTL)活性、白介素2(IL-2)产生情况、细胞毒T淋巴细胞(T淋巴细胞)的产生情况T细胞产生干扰素。我们分析了T细胞中的CD4部分和CD8部分。我们认为肿瘤抗原肽被抗原提呈细胞(APC)捕获，辅助T细胞被激活，CTL也被进一步激活。仅接种肿瘤抗原肽后，我们看不到CTL的激活，但接种肿瘤抗原肽和MDP后，我们可以看到CTL的激活。我们不能确定来自植物乳杆菌的抗原肽的抗肿瘤作用。

After we transplanted P815 mastocytoma to mice, we inoculated tumor antigen peptide (P1A) and the peptide from Lactobacillus plantarum.We investigated survivalrate of those mice.50% survival rate of those mice that were inocurated tumor antigen peptide (P1A) and the peptide from Lactobacillus plantarum was not elongated.Then we inoculated tumor antigen peptide (P1A) and Muramyldipeptide (MDP) after we transplanted P815 mastocytoma to mice.And then we inoculated tumor antigen peptide (P1A) and MDP twice weekly 7 days after we transplanted P815 mastocytoma.We took spleen cell from those mice and took up T cell.We investigated cytotoxic T lymphocyte (CTL) activity, IL-2 production, IFN-rproduction of T cell.And we analized CD4 portion and CD8 portion from T cell.We thought that tumor antigen peptide was catched by Antigen presenting cell (APC), helper Tcell was activated and CTL was also activated further more.We could not see CTL activation after we inoculated only tumor antigen peptide.But we could see CTL activation after we inoculated tumor antigen peptide and MDP.We could not make sure anti-tumor effect of the peptide from Lactobacillus plantarum.