Anti-tumor effect and mechanism of the peptide from Lactobacillus plantarum

植物乳杆菌肽的抗肿瘤作用及机制

• 批准号: 09672045
• 负责人: AMINO Kayoko
• 金额: $ 1.98万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09672045/
• 关键词: Tumor antigen peptide; CTL activity; Muramyl dipeptide (MDP); 腫瘍抗原ペプチド(PIA); 癌抗原ペプチド; Muramyldipeptide(MDP)

After we transplanted P815 mastocytoma to mice, we inoculated tumor antigen peptide (P1A) and the peptide from Lactobacillus plantarum.We investigated survivalrate of those mice.50% survival rate of those mice that were inocurated tumor antigen peptide (P1A) and the peptide from Lactobacillus plantarum was not elongated.Then we inoculated tumor antigen peptide (P1A) and Muramyldipeptide (MDP) after we transplanted P815 mastocytoma to mice.And then we inoculated tumor antigen peptide (P1A) and MDP twice weekly 7 days after we transplanted P815 mastocytoma.We took spleen cell from those mice and took up T cell.We investigated cytotoxic T lymphocyte (CTL) activity, IL-2 production, IFN-rproduction of T cell.And we analized CD4 portion and CD8 portion from T cell.We thought that tumor antigen peptide was catched by Antigen presenting cell (APC), helper Tcell was activated and CTL was also activated further more.We could not see CTL activation after we inoculated only tumor antigen peptide.But we could see CTL activation after we inoculated tumor antigen peptide and MDP.We could not make sure anti-tumor effect of the peptide from Lactobacillus plantarum.

我们将P815肥大细胞瘤移植到小鼠体内后，将肿瘤抗原肽（P1 A）和植物乳杆菌肽（MDP）分别接种于小鼠体内，观察小鼠的存活率，结果表明，接种肿瘤抗原肽（P1 A）的小鼠存活率为50%，而植物乳杆菌肽（MDP）的小鼠存活率为50%，接种肿瘤抗原肽（P1 A）的小鼠存活率为50%，而植物乳杆菌肽（MDP）的小鼠存活率为50%，接种肿瘤抗原肽（P1 A）的小鼠存活率为50%，而植物乳杆菌肽的小鼠存活率为50%，接种肿瘤抗原肽（P1 A）的小鼠存活率为50%，而植物乳杆菌肽的小鼠存活率为50%。方法：将P815肥大细胞瘤移植于小鼠体内，于移植瘤后7天每周2次接种肿瘤抗原肽（P1 A）和MDP，取脾细胞提取T细胞，检测细胞毒性T淋巴细胞（CTL）活性、IL-2产生、对T细胞产生IFN-γ的能力进行了研究，并对T细胞的CD_4和CD_8部分进行了分析，认为肿瘤抗原肽被抗原呈递细胞（APC）捕获，结果表明，肿瘤抗原肽能激活辅助性T细胞，并进一步激活CTL，单纯接种肿瘤抗原肽后，未见CTL的激活，但接种肿瘤抗原肽后，肿瘤抗原肽与MDP联合接种后，可观察到CTL的激活，尚不能确定植物乳杆菌肽的抗肿瘤作用。

项目成果

Tadahiko Kawai, Masayoshi Sakuda et al.: "Diagnostic imaging for a case of maxillary myxoma with a review of the magnetic resonance images of myxoid lesions" Oral Surg Oral Pathol Oral Radiol Endod. 84・4. 449-454 (1997)

Tadahiko Kawai、Masayoshi Sakuda 等：“上颌粘液瘤病例的诊断成像与粘液样病变的磁共振图像的回顾” Oral Surg Oral Pathol Oral Radiol Endod 84・4 (1997)。

Masayoshi Sakuda: "The situation of clinical practice of undergraduate-education and valuation on operation skill and behavior in oral surgery" The Journal of Japanese Association for Dental education. 13・1. 113-120 (1997)

作田正义：“口腔外科本科教育的临床实践状况和操作技能和行为的评估”日本牙科教育协会杂志13・1（1997）。

Tadahiko Kawai, Kayoko Amino et al.: "Mucus escape reaction that involves the mandible" Oral Surg Oral Pathol Oral Radiol Endod. 83・3. 408-415 (1997)

Tadahiko Kawai、Kayoko Amino 等：“涉及下颌骨的粘液逸出反应”Oral Surg Oral Pathol Oral Radiol Endod 83・3（1997）。

作田正義: "口腔外科の卒前臨床実習の現状と口腔外科領域における技能・態度に関する評価" 日本歯科医学教育学会雑誌. 13(1). 113-120 (1997)

Masayoshi Sakuta：“口腔外科研究生临床培训的现状以及口腔外科领域的技能和态度的评估”，日本牙科教育学会杂志 13(1) (1997)。

Tadahiko Kawai: "Mucus escape reaction that involves the mandible" ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY. 83・3. 408-15 (1997)

河井忠彦：“涉及下颌骨的粘液逃逸反应”口腔外科口腔医学口腔病理学 83・3（1997）。